Video: Vim li cas koj thiaj xav tau qhov khoob hauv spectrophotometer?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
A dawb paug cuvette yog siv los calibrate lub spectrophotometer nyeem: lawv sau cov ntsiab lus teb ntawm ib puag ncig-cov cuab yeej-cov qauv qauv. Nws yog ib qho piv txwv rau "zeroing" ib teev ua ntej hnyav. Khiav a dawb paug tso cai koj los sau cov kev cuam tshuam ntawm cov cuab yeej tshwj xeeb ntawm koj cov ntawv nyeem.
Hais txog qhov no, yog vim li cas yog ib qho dawb paug tsim nyog nyob rau hauv ib tug spectrophotometer?
Spectrophotometer yuav tsum tau calibrated tiv thaiv a dawb paug tov kom ntsuas tom qab nws siv tau cov dawb paug daws qhov absorbance raws li xoom siv. Ib qho kev ntsuas ntawm lub peev xwm ntawm ib yam khoom los nqus lub teeb ntawm ib lub teeb wavelength. Nws yog sib npaug rau lub logarithm ntawm lub reciprocal ntawm lub transmittance.
Tom qab ntawd, lo lus nug yog, dab tsi yog qhov dawb paug spectrophotometer? A dawb paug yog ib qho piv txwv uas muaj txhua yam tshwj tsis yog rau kev txheeb xyuas ntawm kev txaus siab. Piv txwv li, yog tias koj tab tom ua qhov kev sim UV-vis los ntsuas qhov concentration ntawm Green Fluorescent Protein, cov protein yuav tsum tau yaj hauv cov kuab tshuaj. Cov dawb paug yog ib qho piv txwv ntawm cov kuab tshuaj xwb.
Dab tsi yog kev siv qhov khoob hauv kev kwv yees colorimetric?
A dawb paug kev daws yog ib qho kev daws teeb meem uas muaj me me los tsis muaj kev txheeb xyuas ntawm kev txaus siab, feem ntau siv mus calibrate cov cuab yeej xws li ib tug colorimeter.
Ib qho qauv dawb yog dab tsi?
Ib tug reagent dawb paug hais txog qhov ua yuam kev me me hauv cov txiaj ntsig kev sim uas los ntawm cov reagents lawv tus kheej. A qauv dawb hais txog kev siv cov qauv rau zeroing ib qho cuab yeej thaum lub sij hawm xeem. A qauv dawb tuaj yeem kho rau qhov yuam kev los ntawm cov xim uas twb muaj lawm lossis turbidity hauv cov qauv ua ntej reagents ntxiv.
Pom zoo:
Vim li cas peb thiaj siv qhov khoob hauv spectrophotometer?
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