Cov txheej txheem:

Dab tsi yog gene cloning siv rau?
Dab tsi yog gene cloning siv rau?

Video: Dab tsi yog gene cloning siv rau?

Video: Dab tsi yog gene cloning siv rau?
Video: Npawg Xeeb thiab Maiv Xis part2 Dab neeg Dab 4/28/2020 2024, Kaum ib hlis
Anonim

Gene cloning yog ib qho kev coj ua hauv molecular biology labs uas yog siv los ntawm cov kws tshawb fawb los tsim cov ntawv luam ntawm ib qho tshwj xeeb noob rau cov ntawv sau qis, xws li kev sib txuas, mutagenesis, genotyping lossis heterologous qhia ntawm cov protein.

Ib yam li ntawd, gene cloning pab tau li cas?

Cloning noob tuaj yeem yog pab tau hauv kev kho thiab kho caj ces Cov kab mob xws li cystic fibrosis thiab mob hnyav ua ke tiv thaiv kab mob (SCID). Thawj theem ntawm cloning ib noob yog tsim kom muaj ib qho DNA seem uas muaj cov noob ntawm kev txaus siab yuav cloned.

Tom qab, lo lus nug yog, gene cloning ua li cas? Nyob rau hauv ib txwm DNA cloning txheej txheem, cov noob lossis lwm yam DNA feem ntawm kev txaus siab (tej zaum a noob rau kev kho mob tseem ceeb ntawm tib neeg cov protein) yog thawj zaug tso rau hauv ib ncig ntawm DNA hu ua plasmid. Thaum lawv rov tsim dua, lawv rov ua dua cov plasmid thiab xa mus rau lawv cov xeeb ntxwv, ua cov ntawv luam ntawm DNA nws muaj.

Hauv qhov no, DNA cloning siv rau dab tsi?

DNA cloning yog siv rau tsim ntau cov ntawv luam ntawm cov noob caj noob ces lossis lwm daim DNA . Cov cloned DNA tuaj yeem yog siv rau : Ua haujlwm ntawm cov noob. Tshawb nrhiav cov yam ntxwv ntawm cov noob (qhov loj me, kev qhia, kev faib cov ntaub so ntswg)

Dab tsi yog 4 kauj ruam ntawm noob cloning?

Nyob rau hauv classical txwv enzyme digestion thiab ligation cloning raws tu qauv, cloning ntawm tej DNA fragment yeej tseem ceeb muaj plaub kauj ruam:

  • Kev cais tawm ntawm DNA ntawm kev txaus siab (lossis lub hom phiaj DNA),
  • kev ligation,
  • transfection (los yog transformation), thiab.
  • ib txoj kev tshuaj ntsuam/xaiv.

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