DNA cloning siv rau dab tsi?
DNA cloning siv rau dab tsi?

Video: DNA cloning siv rau dab tsi?

Video: DNA cloning siv rau dab tsi?
Video: Dab tuag tsev neeg nyob twj cuab 12-13-2019 2024, Kaum ib hlis
Anonim

DNA cloning yog siv tsim kom muaj ntau cov ntawv luam ntawm cov noob caj noob ces lossis lwm daim ntawm DNA . Cov cloned DNA tuaj yeem yog siv rau: Ua haujlwm tawm ntawm cov gene. Tshawb nrhiav cov yam ntxwv ntawm cov noob (qhov loj me, kev qhia, kev faib cov ntaub so ntswg)

Tsis tas li ntawd, lub hom phiaj ntawm cloning yog dab tsi?

Kev kho mob cloning koom nrog tsim ib cloned embryo rau ib leeg lub hom phiaj ntawm kev tsim cov embryonic qia hlwb nrog tib DNA raws li tus pub cell. Cov qia hlwb no tuaj yeem siv rau hauv kev sim ua kom nkag siab txog kab mob thiab tsim kho tshiab rau cov kab mob.

Ib sab saum toj no, DNA cloning ua haujlwm li cas? DNA cloning yog tus txheej txheem ntawm kev ua ntau yam, zoo tib yam luam ntawm ib daim ntawm DNA . Qhov ntxig yog ua tiav siv cov enzymes uas "txiav thiab muab tshuaj txhuam" DNA , thiab nws tsim ib tug molecule ntawm recombinant DNA , los yog DNA sib sau los ntawm cov khoom tawg los ntawm ntau qhov chaw.

Ib yam li ntawd, nws tau nug, dab tsi yog cov txiaj ntsig ntawm DNA cloning?

Ib qho tseem ceeb tshaj plaws pab ntawm DNA cloning thiab genetic engineering rau cell biology yog tias lawv tau ua kom tsim tau ib qho ntawm cov cell proteins nyob rau hauv ze li ntawm unlimited nyiaj. Cov protein ntau uas xav tau yog tsim nyob rau hauv lub hlwb los ntawm kev siv cov vectors (Daim duab 8-42).

Dab tsi yog ob txoj kev siv los clone DNA?

Muaj ob hom gene cloning : nyob rau hauv vivo, uas koom nrog kev siv cov kev txwv enzymes thiab ligases siv vectors thiab cloning cov fragments rau hauv host cells (raws li tau pom hauv daim duab saum toj no). Lwm hom yog nyob rau hauv vitro uas yog siv cov txheej txheem polymerase saw cov tshuaj tiv thaiv (PCR) los tsim cov ntawv luam ntawm cov khoom tawg. DNA.

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