Video: Dab tsi yog Masson trichrome stain?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Masson's trichrome yog peb xim staining raws tu qauv siv nyob rau hauv histology. Nws yog siv rau stain cov nuclei. Tshuaj A, kuj hu ua plasma stain , muaj cov kua qaub fuchsin, Xylidine Ponceau, glacial acetic acid, thiab dej distilled. Lwm cov xim liab acid dyes ua tau siv, np. Biebrich scarlet nyob rau hauv Lillie's trichrome.
Tom qab ntawd, lub hom phiaj ntawm trichrome stain yog dab tsi?
Trichrome staining yog histological staining txoj kev uas siv ob los yog ntau tshaj acid dyes ua ke nrog ib tug polyacid. Kev pleev xim distinguishes cov ntaub so ntswg los ntawm tinting lawv nyob rau hauv contrasting xim. Ib txhia trichrome staining cov kev cai yog Masson's trichrome stain , Lili's trichrome , thiab Gömöri trichrome stain.
Tsis tas li ntawd, xim dab tsi collagen stain? Collagen fibers stain ntsuab los yog xiav nrog Masson's trichrome stain. Cov nqaij thiab keratin yuav liab.
Tsis tas li ntawd nug, dab tsi cov leeg nqaij tau txais hauv Masson trichrome staining?
Masson Trichrome Staining Weigert's hlau hematoxylin stains lub nuclei dub, Biebrich scarlet-acid fuchsin stains cytoplasm thiab cov leeg fibers hauv liab thiab tom qab kho nrog phosphotungstic thiab phosphomolybdic acid, collagen yog ua qias neeg hauv xiav nrog aniline xiav. Kev pleev xim txheej txheem: 1.
Reticulin stain rau dab tsi?
Cov reticulin stain yog dav siv nyob rau hauv lub histopathology kuaj rau staining daim siab cov qauv, tab sis kuj tuaj yeem siv los txheeb xyuas fibrosis hauv cov pob txha pob txha core biopsy specimens. Tsis tas li ntawd, fibrosis tuaj yeem pom ntawm cov pob txha pob txha uas muaj cov qog nqaij hlav loj heev.
Pom zoo:
Nile liab stain yog dab tsi?
Nile liab (tseem hu ua Nile xiav oxazone) yog lipophilic stain. Nile liab stains intracellular lipid droplets daj. Nile liab kuj tau siv los ua ib feem ntawm cov txheej txheem tshawb nrhiav kom pom tseeb rau microplastics hauv lub raj mis dej
Cov xim dab tsi yog cov hlwb feem ntau ua ntej siv thawj cov xim rau cov txheej txheem Gram stain?
Ua ntej, siv lead ua violet, thawj stain, yog siv rau lub tshav kub-tso smear, muab tag nrho cov hlwb xim liab
Dab tsi yog qhov tseeb lom neeg stain?
Cov kab mob lom neeg yog hais txog cov khoom sib xyaw uas hloov cov xim ntawm cov yam ntxwv ntawm cov cell xws li phab ntsa ntawm tes lossis cov nucleus ntawm lub xov tooj ntawm tes thiab pab saib lawv kom pom tseeb dua. Lub kas fes stain tsis ua li ntawd. Thaum koj siv cov kua qaub-cawv, nws decolorizes lub hlwb thiab cov stain raug tshem tawm
Dab tsi yog qhov txhais ntawm ib qho yooj yim stain?
Qhov yooj yim stain tuaj yeem siv los txiav txim siab cov duab ntawm tes, qhov loj me, thiab kev teeb tsa. Muaj tseeb rau nws lub npe, qhov yooj yim stain yog ib qho yooj yim staining txheej txheem uas tsuas yog ib tug stain. Cov stains no yuav yooj yim tso ib qho hydroxide ion los yog txais ib qho hydrogen ion, uas tawm ntawm qhov stain zoo
Dab tsi yog qhov tseem ceeb tshaj plaws reagent nyob rau hauv txoj kev Gram stain?
Lub ntsiab stain ntawm Gram txoj kev yog siv lead ua violet. Crystal violet qee zaum hloov nrog methylene xiav, uas zoo sib xws. Cov kab mob uas khaws cia cov siv lead ua violet-iodine complex tshwm ntshav xim av nyob rau hauv kev tshuaj xyuas microscopic