Cov txheej txheem:

Dab tsi yog qhov tseem ceeb tshaj plaws reagent nyob rau hauv txoj kev Gram stain?
Dab tsi yog qhov tseem ceeb tshaj plaws reagent nyob rau hauv txoj kev Gram stain?

Video: Dab tsi yog qhov tseem ceeb tshaj plaws reagent nyob rau hauv txoj kev Gram stain?

Video: Dab tsi yog qhov tseem ceeb tshaj plaws reagent nyob rau hauv txoj kev Gram stain?
Video: Yog ntuj tsis pab ntshe kuv tuag lawm 8/6/2018 2024, Kaum ib hlis
Anonim

Lub ntsiab stain ntawm Gram txoj kev yog siv lead ua violet . Crystal violet qee zaum hloov nrog methylene xiav, uas zoo sib xws. Cov microorganisms uas khaws cov siv lead ua violet - iodine complex tshwm liab doog xim av nyob rau hauv microscopic kuaj.

Xav txog qhov no, dab tsi yog reagent hauv Gram staining?

Cov Gram Stain reagents yog siv los txiav txim siab Gram cov tshuaj tiv thaiv rau kev txheeb xyuas kab mob. Crystal Violet stains kab mob cell. Iodine , lub mordant, khi lub stain. Cawv- acetone tshuaj, tus decolorizer, sib txawv cov kab mob los ntawm kev tuav los yog tsis siv lead ua violet , nyob rau hauv lawv lub cell phab ntsa.

Ib yam li ntawd, vim li cas decolorization tseem ceeb hauv Gram staining? nthuav tawm grams Cov hlwb tsis zoo rau cov decolorizer dissolves lipids nyob rau hauv cov phab ntsa ntawm tes, uas tso cai rau cov siv lead ua violet-iodine complex rau leach tawm ntawm lub hlwb. Qhov no tso cai rau cov hlwb tom qab ua qias neeg nrog safranin.

Ntawm no, Gram staining yuav pab tau li cas hauv kev kho mob?

Qhov txiaj ntsig tseem ceeb ntawm a gram stain yog tias nws pab koj tus kws kho mob kawm yog tias koj muaj kab mob, thiab nws txiav txim siab seb hom kab mob twg ua rau nws. Qhov no ua tau pab koj tus kws kho mob txiav txim siab txog txoj kev kho mob zoo.

Dab tsi yog cov kauj ruam ntawm Gram staining?

Muaj plaub theem pib ntawm Gram stain:

  1. Siv cov xim tseem ceeb (crystal violet) rau lub tshav kub-tso smear ntawm cov kab mob kab mob.
  2. Qhov sib ntxiv ntawm iodide, uas khi rau crystal violet thiab ntxiab nws nyob rau hauv lub cell.
  3. Kev tshem tawm sai sai nrog ethanol lossis acetone.
  4. Counterstaining nrog safranin.

Pom zoo: