Cov phaj hauv chromatography yog dab tsi?
Cov phaj hauv chromatography yog dab tsi?

Video: Cov phaj hauv chromatography yog dab tsi?

Video: Cov phaj hauv chromatography yog dab tsi?
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Daim phiaj yog generated thaum lub sij hawm elution ntawm solutes los ntawm a chromatographic kem thiab muaj ib tug nplua nuj ntawm cov ntaub ntawv hais txog cov txheej txheem sib cais, feem ntau ncov dispersion. Nws yog ib qho yooj yim ntsuas kom muaj nuj nqis siv los soj ntsuam cov khoom.

Hais txog qhov no, lo lus theoretical phaj txhais li cas?

theoretical - phaj . Noun. (plural theoretical daim hlau ) (Physics, Chemistry) A phaj los yog tais nyob rau hauv ib tug distillation kem uas ua tau qhov zoo tshaj plaws sib txawv ntawm cov kua thiab vapor theem nyob rau hauv equilibrium nrog nws; lub tswvyim sib npaug nyob rau hauv ib kem fractionating packed nrog hlaws dai los yog rings.

Tsis tas li, dab tsi yog txhais los ntawm cov phiaj xwm theoretical hauv HPLC? A theoretical phaj nyob rau hauv ntau txoj kev sib cais yog ib cheeb tsam hypothetical los yog theem nyob rau hauv uas ob theem, xws li cov kua thiab vapor theem ntawm ib yam khoom, tsim kom muaj ib tug equilibrium. Cov theem sib npaug no kuj tseem raug xa mus rau theem sib npaug, theem zoo tagnrho, lossis theoretical tais.

Kuj nug, lub phaj siab yog dab tsi?

Phaj qhov siab . Hauv chromatography, ncov dav nce hauv kev faib ua feem rau lub hauv paus square ntawm qhov deb uas lub ncov tau tsiv mus. Cov qhov siab sib npaug rau qhov theoretical phaj , raws li tau hais los saum toj no, txhais tau tias yog proportionality tas li hais txog tus qauv sib txawv thiab qhov kev ncua deb mus.

Hetp suav li cas?

Phaj Height Chromatography Formula Koj tuaj yeem siv qhov "qhov siab sib npaug rau lub phaj theoretical" ( HETP ) hauv equation HETP = A + B / v + Cv rau Eddy-diffusion term A, longitudinal diffusion lub sij hawm B, tsis kam mus rau huab hwm coj hloov coefficient C thiab linear tshaj tawm v.

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