Cov txheej txheem:
Video: Koj npaj li cas van Giison stain?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Txoj kev
- 1 Nqa cov seem rau cov dej distilled.
- 2 Stain nuclei nrog Celestin Blue 5 mins.
- 3 Yaug hauv dej distilled.
- 4 Stain hauv hematoxylin 5 mins.
- 5 Ntxuav kom zoo hauv dej kais dej 5 feeb.
- 6 Dej nyab nrog Curtis stain 5 feeb.
- 7 Blot.
- 8 Lub cev qhuav dej sai sai hauv cov cawv, ntshiab thiab mount.
Hauv qhov no, Van Giison stain siv rau dab tsi?
Van Gieson Stain yog siv kom sib txawv ntawm collagen thiab cov nqaij mos hauv cov qog thiab ua kom pom qhov nce ntawm collagen hauv cov kab mob. Txoj kev no muab ob lossis ntau dua anionic dyes thiab tso siab rau qhov sib txawv los ntawm cov ntaub so ntswg.
Ib tug kuj nug, dab tsi xim HVG stain collagen? xiav
Cov lus nug tseem yog, Masson's Trichrome stain siv rau dab tsi?
Standard applications: Masson's trichrome staining yog dav siv rau kawm txog cov nqaij mos pathologies (muscular dystrophy), mob plawv pathologies (infarct), kab mob siab (cirrhosis) lossis raum pathologies (glomerular fibrosis). Nws kuj tuaj yeem ua tau siv rau tshawb xyuas thiab txheeb xyuas cov qog nqaij hlav ntawm lub siab thiab lub raum biopsies.
H thiab E staining ua haujlwm li cas?
H&E muaj ob dyes haemotoxylin thiab eosin. Eosin yog ib qho acidic dye: nws raug nqi tsis zoo (cov qauv dav dav rau acidic dyes yog: Na+xim-). Nws stains yooj yim (los yog acidophilic) qauv liab lossis liab. Yog li lub nucleus yog ua qias neeg ntshav hauv daim duab hauv qab no, los ntawm H&E staining.
Pom zoo:
Cov pob zeb sedimentary tau npaj li cas?
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