Cov txheej txheem:
Video: Cov khoom ntawm PCR yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov ntsiab lus tseem ceeb ntawm PCR cov tshuaj tiv thaiv muaj xws li a DNA template, primers, nucleotides, DNA polymerase, thiab tsis muaj. Cov DNA template feem ntau yog koj tus qauv DNA , uas muaj cov DNA cheeb tsam yuav tsum tau amplified.
Hauv qhov no, PCR piav qhia nws cov khoom thiab cov txheej txheem yog dab tsi?
Cov polymerase saw cov tshuaj tiv thaiv ( PCR ) yog cov txheej txheem kuaj rau DNA rov ua dua uas tso cai rau "lub hom phiaj" DNA sib lawv liag los xaiv kom nthuav dav. PCR tuaj yeem siv cov qauv me tshaj plaws ntawm DNA los ua cloned thiab nthuav dav rau ntau lab luam hauv ob peb teev xwb.
Ib yam li ntawd, PCR template yog dab tsi? Polymerase chain reaction , los yog PCR , yog cov txheej txheem kuaj siv los ua ntau cov ntawv luam ntawm ib ntu ntawm DNA. Ces, ua PCR , cov DNA tus qauv uas muaj lub hom phiaj yog ntxiv rau lub raj uas muaj primers, dawb nucleotides, thiab ib tug enzyme hu ua DNA polymerase, thiab cov sib tov yog muab tso rau hauv ib tug PCR tshuab.
Ntawm no, plaub yam tseem ceeb ntawm PCR DNA amplification cov tshuaj tiv thaiv yog dab tsi?
DNA Template, Cov DNA Polymerase , Oligonucleotide Primers, thiab Nucleotides.
4 kauj ruam ntawm PCR yog dab tsi?
Cov kauj ruam koom nrog hauv Polymerase Chain Reaction hauv DNA Sequence
- Kauj Ruam 1: Denaturation los ntawm Thaum tshav kub kub: Thaum tshav kub kub yog feem ntau ntau tshaj 90 degrees Celsius ntawm cais ob-stranded DNA rau hauv ob qho tib si strands.
- Kauj Ruam 2: Annealing Primer rau Target Sequence:
- Kauj Ruam 3: Extension:
- Kauj Ruam 4: Xaus ntawm Thawj PGR voj voog:
Pom zoo:
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