Yuav ua li cas silica gel npaj rau kem chromatography?
Yuav ua li cas silica gel npaj rau kem chromatography?

Video: Yuav ua li cas silica gel npaj rau kem chromatography?

Video: Yuav ua li cas silica gel npaj rau kem chromatography?
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Kev npaj ntawm Kem :

Npaj slurry ntawm silica gel nrog ib tug haum hnyav & ncuav maj mam rau hauv lub kem . Qhib qhov nres qaib & cia qee qhov hnyav kom ntws tawm. Txheej ntawm cov kuab tshuaj yuav tsum nco ntsoov npog cov adsorbent; txwv tsis pub cov kab nrib pleb yuav tsim nyob rau hauv kem

Tsuas yog li ntawd, Silica Gel ua dab tsi hauv kab chromatography?

Silica gel yog ib tug polar adsorbent thiab yog me ntsis acidic nyob rau hauv cov xwm, nws muaj peev xwm nqus tau yooj yim cov ntsiab lus uas tej zaum yuav muaj nyob rau hauv cov khoom uas xav tau kev sib cais los yog purification. Nws kuj tseem paub zoo txog nws lub luag haujlwm hauv kev faib rov qab-theem chromatography.

Qhov thib ob, qhov twg cov khoom sib txuas ua ntej hauv kab chromatography? Cov kuab tshuaj tsawg-polar yog ua ntej siv rau lus tsawg-polar compound . Ib zaug tsawg-polar compound yog tawm ntawm kem , ib qho ntxiv-polar hnyav ntxiv rau hauv kem rau lus qhov ntau-polar compound.

Hais txog qhov no, koj yuav ua li cas npaj cov slurry rau kab chromatography?

Muab 15 mL ntawm hexanes rau hauv 125 mL Erlenmeyer hwj thiab maj mam ntxiv cov hmoov alumina, me ntsis ntawm ib lub sij hawm, thaum swirling. Siv Pasteur pipet los sib tov slurry , ces sai sai pipet lub slurry mus rau ntawm kem (koj tuaj yeem ncuav nws hloov yog tias koj nyiam).

Koj siv silica ntau npaum li cas hauv ib kem?

Qhov ntau zoo yog 100/20 txog 100/10 (5 mus rau 10 mL) ntawm elutant rau 100g silica . Nws tsis yog ib lub tswv yim zoo siv ib qho hnyav sib txawv ntawm no - kev sib cais yuav raug cuam tshuam loj heev.

Pom zoo: