Cov txheej txheem:
Video: Yuav ua li cas koj ntim kab rau kab chromatography?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Packing ib (silica gel) kem:
- Siv ib daim hlau los ntxiv ib lub ntsaws ntawm paj rwb rau hauv qab ntawm lub kem .
- Clamp lub kem mus rau ib lub nplhaib sawv ntsug thiab ntxiv cov xuab zeb txaus los sau qhov nkhaus ntawm qhov kem .
- Muab ib tug pinch clamp rau ntawm lub raj, ces sau lub kem 1/4 mus rau 1/3 tag nrho nrog lub intial eluent.
Ib yam li ib tug yuav nug, ua li cas koj ntim ib kem nrog resin?
Ua tib zoo ncuav lub resin slurry cia rau sab hauv phab ntsa ntawm lub kem . Pouring raws phab ntsa tiv thaiv kom tsis txhob muaj huab cua nyob hauv qhov chaw resin slurry. Tom qab lub resin slurry yog pauv mus rau lub kem , yaug cov phab ntsa sab hauv ntawm lub kem siv lub raj mis squirt uas muaj ntim tsis pub.
Kuj Paub, kab chromatography ua haujlwm li cas? Kem Chromatography yog cov txheej txheem npaj siv los ntxuav cov tebchaw nyob ntawm lawv cov polarity lossis hydrophobicity. Hauv kab chromatography , kev sib xyaw ntawm cov molecules yog sib cais raws li lawv qhov sib txawv ntawm qhov sib txawv ntawm cov theem mobile thiab theem nyob ruaj khov.
Ntawm no, kuv yuav ntim ib kem Sephadex li cas?
Kem packing rau pab pawg sib cais siv Sephadex . Sephadex yog muab raws li cov hmoov qhuav thiab yuav tsum tau tso cai rau o nyob rau hauv ntau buffer ua ntej siv. Tom qab o, kho nrog tsis tuaj yeem tsim cov slurry tuab los ntawm cov cua npuas raug tshem tawm hauv lub tshuab nqus tsev. Kwv yees li ntawm 75% daws qhov nruab nrab yog tsim nyog.
Lub hauv paus ntsiab lus ntawm kab chromatography yog dab tsi?
PRINCIPLE . Lub ntsiab txoj cai koom nrog kab chromatography yog adsorption ntawm cov kuab tshuaj ntawm ib qho kev daws teeb meem los ntawm qhov chaw nyob ruaj khov thiab cais cov sib tov rau hauv ib tus neeg cov khoom. Qhov no yog raws li kev sib raug zoo ntawm lub sijhawm mobile thiab nyob ruaj khov.
Pom zoo:
Yuav ua li cas koj thauj cov qauv hauv kab chromatography?
Txhawm rau thauj cov kab: Dissolve cov qauv hauv qhov tsawg kawg nkaus ntawm cov kuab tshuaj (5-10 tee). Siv lub pipette los yog syringe nrog ib rab koob tuab, drip tus qauv ncaj qha mus rau sab saum toj ntawm silica. Thaum tag nrho cov qauv tau ntxiv lawm, cia cov kem kom ntws tawm kom cov qib hnyav kov rau saum cov silica
Yuav ua li cas hloov lub ntim ntim hauv tsev muag tshuaj?
Kev hloov pauv ntawm cov tshuaj X yog 0.5mL / 40mg. Yog hais tias qhov xav tau concentration yog 4mg hauv 1mL, ces 20mL yog xav tau rau 80mg ntawm tshuaj X. Yog hais tias 40mg displaces 0.5mL ntawm cov tshuaj, nws txhais tau tias 80mg displaces 1mL. 20mL - 1mL = 19mL ntawm diluent xav tau
Yuav ua li cas koj mus ntawm moles rau ntim?
Hloov los ntawm moles rau ntim (litres): Ua kom koj tus nqi mole los ntawm molar volumeconstant, 22.4L. Hloov los ntawm cov khoom (atoms, molecules, orformula units) rau moles: Faib koj cov nqi particle los ntawmAvogadro tus lej, 6.02 × 1023. Nco ntsoov siv cov kab lus ntawm koj lub laij lej
Nws puas yuav ua rau pom qhov sib npaug ntawm kab sib npaug mus rau ib kab thiab los ntawm ib qho taw tes ntawm kab?
Qhov sib npaug ntawm ib kab uas yog parallel lossis perpendicular rau ib kab? Cov lus teb tau: Cov nqes hav ntawm cov kab sib npaug sib npaug. Hloov txoj kab nqes paub thiab cov kev sib koom ua ke ntawm ib qho taw tes ntawm lwm txoj kab rau hauv daim ntawv point-slope kom pom qhov sib npaug ntawm kab sib npaug
Yuav ua li cas muaj pes tsawg tus tooj liab atoms koj yuav tsum tau kab ntawm ib sab los tsim ib kab 1'm ntev?
Los ntawm kev sib piv, lub ntiaj teb cov pej xeem tsuas yog hais txog 7? 109 neeg. Yog tias koj tuaj yeem ua kab 100,000,000 tooj liab atoms ib sab, lawv yuav tsim ib txoj kab ntev li 1 cm