Video: Dab tsi yog kev qhia mutagenesis?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Qhia mutagenesis , kuj hu ua qhia kev hloov pauv , yog ib qho kev xav hais tias cov kab mob tuaj yeem teb rau ib puag ncig kev ntxhov siab los ntawm orthogenetically qhia Kev hloov pauv rau qee cov noob lossis thaj chaw ntawm genome.
Ib yam li ib tug yuav nug, lub hom phiaj ntawm qhov chaw qhia mutagenesis yog dab tsi?
Qhov chaw - qhia mutagenesis yog ib txoj hauv kev molecular biology uas yog siv los ua qhov tshwj xeeb thiab txhob txwm hloov pauv mus rau DNA ib ntu ntawm cov noob thiab txhua yam khoom siv noob.
Tsis tas li ntawd, koj ua li cas tsim ib qhov chaw qhia mutagenesis primer? Ob tug primers yuav tsum tau tsim nyob rau hauv opposite lus qhia nrog lawv 5 'kawg nyob ib sab ntawm lub cheeb tsam yuav tsum tau muab tshem tawm. Cov primers tuaj yeem ua tau 100% ntxiv rau plasmid ib ntus lossis tuaj yeem muaj qhov tsis sib xws thiab / lossis kev ntxig yog xav tau. Qhov sib lawv liag yuav tsum tau muab ntxiv rau 5' kawg ntawm qhov mutagenic primer.
Ntawm no, qhov chaw qhia mutagenesis ua li cas?
Qhov chaw - qhia mutagenesis (SDM) yog ib txoj hauv kev los tsim cov kev hloov pauv tshwj xeeb hauv ob chav plasmid DNA. Txhawm rau kawm txog kev hloov pauv hauv cov haujlwm protein uas tshwm sim los ntawm DNA kev tswj hwm. Txhawm rau xaiv lossis tshuaj ntsuam kev hloov pauv (ntawm DNA, RNA lossis qib protein) uas muaj cov khoom xav tau.
PCR mutagenesis yog dab tsi?
PCR mutagenesis yog ib txoj hauv kev los tsim qhov chaw qhia mutagenesis . Txoj kev no tuaj yeem tsim cov kev hloov pauv (piv txwv, ntxig, thiab tshem tawm) los ntawm ob npaug-stranded plasmid yam tsis tas yuav tsum muaj subcloning rau hauv M13-based bacteriophage vectors thiab rau ssDNA cawm.
Pom zoo:
Dab tsi yog qhov txiaj ntsig tseem ceeb ntawm kev taw qhia ntawm tes los ntawm kev sib cuag lub cev ncaj qha?
Kev taw qhia kuj tshwm sim ntawm cov hlwb uas yog kev sib cuag ntawm lub cev. Kev sib cuam tshuam ntawm cov proteins ntawm qhov chaw ntawm cov hlwb tuaj yeem ua rau muaj kev hloov pauv hauv cov cwj pwm ntawm tes. Piv txwv li, cov proteins nyob rau saum npoo ntawm T-cells thiab antigen nthuav tawm cov hlwb sib cuam tshuam los ua kom muaj kev taw qhia hauv T-cells
Dab tsi yog qhov txawv ntawm kev xaiv kev taw qhia thiab kev xaiv cuam tshuam?
Hauv kev xaiv cov kev taw qhia, cov pej xeem cov noob caj noob ces hloov mus rau qhov tshiab phenotype thaum raug kev hloov pauv ib puag ncig. Hauv kev sib txawv lossis kev cuam tshuam kev xaiv, qhov nruab nrab lossis nruab nrab phenotypes feem ntau tsis haum dua li qhov phenotype huab cua thiab tsis zoo li yuav ua rau muaj txiaj ntsig zoo hauv cov pej xeem
Kev taw qhia ntawm daim ntawv qhia yog dab tsi?
Kev taw qhia ntawm daim ntawv qhia yog kev sib raug zoo ntawm cov lus qhia ntawm daim ntawv qhia thiab cov lus qhia sib piv hauv kev muaj tiag. Lo lus 'orient' yog muab los ntawm Latin oriens, txhais tau tias sab hnub tuaj. Qhov feem ntau cartographic convention, yog tias sab qaum teb yog nyob rau sab saum toj ntawm daim ntawv qhia
Dab tsi yog ib qho kev qhia uas muaj tsawg kawg yog ib qho kev sib txawv?
Algebraic qhia ?: Cov lus lej suav nrog tsawg kawg ib qho sib txawv thiab qee zaum tus lej thiab cov cim ua haujlwm
Dab tsi yog qhov kev piav qhia zoo tshaj plaws ntawm lub ntiaj teb nebula yog dab tsi?
Planetary nebula yog ib qho khoom siv hnub qub uas muaj lub plhaub ci ntsa iab ntawm cov pa roj thiab cov ntshav uas tsim los ntawm qee hom hnub qub thaum kawg ntawm lawv lub neej. Lawv nyob rau hauv qhov tseeb tsis cuam tshuam rau cov ntiaj chaw; lub npe originates los ntawm ib tug supposed zoo sib xws nyob rau hauv tsos rau giant ntiaj chaw