Kev ntsuam xyuas lysozyme enzyme yog dab tsi?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Tus qauv soj ntsuam mus kuaj rau lub kev ua haujlwm enzymatic ntawm lysozyme yog qhov txo qis turbidity soj ntsuam . Ntawm no, qhov txo qis hauv OD ntawm Micrococcus lysodeikticus tov ntawm 450 nm yog ntsuas raws sijhawm. Txawm li cas los, nyob rau hauv cov ntaub ntawv, lub kev ua haujlwm enzymatic ntawm zoo sib xws cov enzymes feem ntau ntsuas ntawm 600 nm.

Tom qab ntawd, ntsuas kev ua lysozyme li cas?

Kev ua haujlwm lysozyme yog ntsuas ntawm OD 450 los ntawm kev taug qab qhov txo qis hauv qhov muag pom qhov muag raws li lub substrate lub cell phab ntsa tawg los ntawm cov enzyme yog tias nws muaj. Ceev nrooj ntxiv 0.1 ml ntawm cov lysozyme , sib tov los ntawm inverting lub cuvette, thiab tam sim ntawd muab nws rov qab rau hauv lub spectrophotometer.

Ib yam li ntawd, koj ua li cas xam cov kev ua enzyme? Kev ua haujlwm enzyme = moles ntawm substrate hloov dua siab tshiab rau ib chav tsev lub sij hawm = tus nqi × cov tshuaj tiv thaiv ntim. Kev ua haujlwm enzyme yog a ntsuas ntawm qhov ntau ntawm active enzyme tam sim no thiab yog li nyob ntawm tej yam kev mob, uas yuav tsum tau teev. SI unit yog katal, 1 katal = 1 mol s⁻¹, tab sis qhov no yog ib lub tsev loj heev.

Ntawm no, lysozyme kev ua haujlwm yog dab tsi?

Lysozyme yog glycoside hydrolase uas catalyzes hydrolysis ntawm 1, 4-beta-kev sib txuas ntawm N-acetylmuramic acid thiab N-acetyl-D-glucosamine residues nyob rau hauv peptidoglycan, uas yog cov feem tseem ceeb ntawm gram-zoo kab mob cell phab ntsa. Qe qe dawb lysozyme tuav nws kev ua si nyob rau hauv ntau yam ntawm pH (6-9).

Yuav ua li cas lysozyme zom cov kab mob cell phab ntsa?

Lysozyme yog ib qho enzyme uas cleaves peptidoglycan hauv kab mob cell phab ntsa los ntawm catalyzing hydrolysis ntawm β-(1, 4) kev sib txuas ntawm NAM thiab NAG saccharides (Fig.