Cov txheej txheem:
Video: Dab tsi yog cov kauj ruam ntawm molecular cloning?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Lub hauv paus cloning workflow muaj plaub kauj ruam:
- Kev rho tawm ntawm lub hom phiaj DNA fragments (feem ntau hu ua inserts)
- Ligation ntawm inserts rau hauv ib qho tsim nyog cloning vector, tsim recombinant molecules (e.g., plasmids)
- Kev hloov pauv ntawm cov plasmids recombinant rau hauv cov kab mob los yog lwm tus tswv tsev tsim nyog rau kev nthuav tawm.
Cov lus nug tseem yog, dab tsi yog 4 kauj ruam ntawm noob cloning?
Nyob rau hauv classical txwv enzyme digestion thiab ligation cloning raws tu qauv, cloning ntawm tej DNA fragment yeej tseem ceeb muaj plaub kauj ruam:
- Kev cais tawm ntawm DNA ntawm kev txaus siab (lossis lub hom phiaj DNA),
- kev ligation,
- transfection (los yog transformation), thiab.
- ib txoj kev tshuaj ntsuam/xaiv.
Qhov thib ob, ua li cas cloning ua ib kauj ruam? Koj phau ntawv qhia rau kev rov ua dua hauv ntiaj teb tiag
- Kauj Ruam 1: Extract DNA los ntawm tus neeg pub dawb.
- Kauj Ruam 2: Npaj ib lub qe qe.
- Kauj Ruam 3: Ntxig cov khoom siv somatic cell.
- Kauj Ruam 4: Cia lub qe tias nws fertilized thiab cog nws.
- Kauj Ruam 5: Rov ua dua kom txog thaum muaj kev vam meej.
Tsis tas li lo lus nug yog, dab tsi yog qhov kev txiav txim ntawm cov xwm txheej rau molecular cloning?
Nyob rau hauv tus qauv molecular cloning kev sim, cov cloning ntawm tej DNA fragment tseem ceeb muaj xya kauj ruam: (1) Kev xaiv ntawm tus tswv tsev thiab cloning vector, (2) Kev npaj ntawm vector DNA , (3) Kev npaj ntawm DNA ua cloned , (4) Tsim cov recombinant DNA , (5) Taw qhia ntawm recombinant DNA nyob rau hauv lub cev, (6)
Dab tsi yog cov kauj ruam hauv DNA recombinant?
Feem ntau, a recombinant DNA technology muaj tsib cov kauj ruam : (1) txiav qhov xav tau DNA los ntawm qhov chaw txwv, (2) nthuav cov noob luam los ntawm PCR, (3) ntxig cov noob rau hauv cov vectors, (4) hloov cov vectors rau hauv lub cev, thiab (5) tau txais cov khoom ntawm recombinant noob (Fig.
Pom zoo:
Cov kauj ruam ntawm cov pa roj carbon monoxide yog dab tsi?
Cov txheej txheem hauv lub voj voog carbon Carbon nkag mus rau hauv huab cua raws li carbon dioxide los ntawm kev ua pa thiab combustion. Cov pa roj carbon dioxide yog absorbed los ntawm cov neeg tsim khoom los ua cov piam thaj hauv photosynthesis. Decomposers rhuav tshem cov kab mob uas tuag lawm thiab xa cov pa roj carbon monoxide hauv lawv lub cev rov qab mus rau qhov chaw zoo li carbon dioxide los ntawm kev ua pa
Dab tsi yog cov kauj ruam los daws qhov tsis sib xws ntawm ob kauj ruam?
Nws yuav siv ob kauj ruam los daws qhov kev sib npaug lossis qhov tsis sib xws uas muaj ntau tshaj ib qho kev ua haujlwm: Simplify siv qhov sib txawv ntawm qhov sib ntxiv lossis rho tawm. Ua kom yooj yim ntxiv los ntawm kev siv qhov sib txawv ntawm qhov sib npaug lossis faib
Cov kauj ruam ntawm mitosis yog dab tsi thiab dab tsi tshwm sim hauv txhua qhov?
Mitosis muaj tsib theem sib txawv: interphase, prophase, metaphase, anaphase thiab telophase. Cov txheej txheem ntawm cell faib tsuas yog ua tiav tom qab cytokinesis, uas tshwm sim thaum lub sij hawm anaphase thiab telophase. Txhua theem ntawm mitosis yog tsim nyog rau cell replication thiab faib
Koj ua li cas electron configuration kauj ruam yog kauj ruam?
Cov kauj ruam Nrhiav koj tus lej atomic. Txiav txim siab tus nqi ntawm lub atom. Nco cov npe ntawm cov orbitals. Nkag siab txog kev teeb tsa hluav taws xob. Nco qhov kev txiav txim ntawm lub orbitals. Sau rau hauv lub orbitals raws li tus naj npawb ntawm electrons hauv koj lub atom. Siv lub sij hawm ntev raws li qhov pom kev luv luv
Dab tsi yog cov kauj ruam ntawm cloning?
Nyob rau hauv classical txwv enzyme digestion thiab ligation cloning raws tu qauv, cloning ntawm tej DNA fragment yeej tseem ceeb muaj plaub kauj ruam: cais DNA ntawm kev txaus siab (los yog phiaj DNA), ligation, transfection (los yog transformation), thiab. ib txoj kev tshuaj ntsuam/xaiv