Cov txheej txheem:

Dab tsi yog cov kauj ruam ntawm molecular cloning?
Dab tsi yog cov kauj ruam ntawm molecular cloning?

Video: Dab tsi yog cov kauj ruam ntawm molecular cloning?

Video: Dab tsi yog cov kauj ruam ntawm molecular cloning?
Video: Koj Hais Dab Tsi?/Suabci Kwm & Ab Meej Ham/Official MV 2023 2024, Kaum ib hlis
Anonim

Lub hauv paus cloning workflow muaj plaub kauj ruam:

  • Kev rho tawm ntawm lub hom phiaj DNA fragments (feem ntau hu ua inserts)
  • Ligation ntawm inserts rau hauv ib qho tsim nyog cloning vector, tsim recombinant molecules (e.g., plasmids)
  • Kev hloov pauv ntawm cov plasmids recombinant rau hauv cov kab mob los yog lwm tus tswv tsev tsim nyog rau kev nthuav tawm.

Cov lus nug tseem yog, dab tsi yog 4 kauj ruam ntawm noob cloning?

Nyob rau hauv classical txwv enzyme digestion thiab ligation cloning raws tu qauv, cloning ntawm tej DNA fragment yeej tseem ceeb muaj plaub kauj ruam:

  • Kev cais tawm ntawm DNA ntawm kev txaus siab (lossis lub hom phiaj DNA),
  • kev ligation,
  • transfection (los yog transformation), thiab.
  • ib txoj kev tshuaj ntsuam/xaiv.

Qhov thib ob, ua li cas cloning ua ib kauj ruam? Koj phau ntawv qhia rau kev rov ua dua hauv ntiaj teb tiag

  1. Kauj Ruam 1: Extract DNA los ntawm tus neeg pub dawb.
  2. Kauj Ruam 2: Npaj ib lub qe qe.
  3. Kauj Ruam 3: Ntxig cov khoom siv somatic cell.
  4. Kauj Ruam 4: Cia lub qe tias nws fertilized thiab cog nws.
  5. Kauj Ruam 5: Rov ua dua kom txog thaum muaj kev vam meej.

Tsis tas li lo lus nug yog, dab tsi yog qhov kev txiav txim ntawm cov xwm txheej rau molecular cloning?

Nyob rau hauv tus qauv molecular cloning kev sim, cov cloning ntawm tej DNA fragment tseem ceeb muaj xya kauj ruam: (1) Kev xaiv ntawm tus tswv tsev thiab cloning vector, (2) Kev npaj ntawm vector DNA , (3) Kev npaj ntawm DNA ua cloned , (4) Tsim cov recombinant DNA , (5) Taw qhia ntawm recombinant DNA nyob rau hauv lub cev, (6)

Dab tsi yog cov kauj ruam hauv DNA recombinant?

Feem ntau, a recombinant DNA technology muaj tsib cov kauj ruam : (1) txiav qhov xav tau DNA los ntawm qhov chaw txwv, (2) nthuav cov noob luam los ntawm PCR, (3) ntxig cov noob rau hauv cov vectors, (4) hloov cov vectors rau hauv lub cev, thiab (5) tau txais cov khoom ntawm recombinant noob (Fig.

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