Cov txheej txheem:
Video: Dab tsi yog cov kauj ruam ntawm cloning?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nyob rau hauv classical txwv enzyme digestion thiab ligation cloning raws tu qauv, cloning ntawm tej DNA fragment yeej tseem ceeb muaj plaub kauj ruam:
- Kev cais tawm ntawm DNA ntawm kev txaus siab (lossis lub hom phiaj DNA),
- kev ligation,
- transfection (los yog transformation), thiab.
- kev tshuaj ntsuam/ xaiv txheej txheem.
Raws li txoj cai, 6 kauj ruam ntawm cloning yog dab tsi?
Nyob rau hauv tus qauv molecular cloning kev sim, cov cloning ntawm ib qho DNA feem tseem ceeb muaj xya cov kauj ruam : (1) Kev xaiv ntawm tus tswv tsev thiab cloning vector, (2) Kev npaj vector DNA, (3) Kev npaj DNA ua cloned , (4) Kev tsim cov DNA recombinant, (5) Kev taw qhia txog DNA recombinant rau hauv lub cev muaj sia, ( 6 )
Ib yam li ntawd, cloning ua haujlwm ib kauj ruam li cas? Koj phau ntawv qhia rau kev rov ua dua hauv ntiaj teb tiag
- Kauj Ruam 1: Extract DNA los ntawm tus neeg pub dawb.
- Kauj Ruam 2: Npaj ib lub qe qe.
- Kauj Ruam 3: Ntxig cov khoom siv somatic cell.
- Kauj Ruam 4: Cia lub qe tias nws fertilized thiab cog nws.
- Kauj Ruam 5: Rov ua dua kom txog thaum muaj kev vam meej.
Yog li ntawd, dab tsi yog qhov txheej txheem ntawm cloning?
Cloning hais txog cov txheej txheem ntawm kev tsim lub embryo nrog DNA los ntawm tus tsiaj laus. Lub embryo tsim tshiab yog ces zapped nrog hluav taws xob kom nws pib multiply, kom txog rau thaum nws dhau los ua blastocyst (ib pawg me me ntawm cov hlwb uas tsim tom qab lub qe yog fertilized), uas yog ces cog rau hauv ib tug surrogate niam.
Ib qho piv txwv ntawm cloning yog dab tsi?
Ib qho classic piv txwv ntawm cov txheej txheem no yog thaum Dolly cov yaj yog cloned nyob rau hauv 1996. Dolly tau tsim siv lub somatic cell los ntawm nws niam. Ib tug somatic cell yog ib tug laus lub cev cell, xws li daim tawv nqaij, plaub hau, los yog nyob rau hauv cov ntaub ntawv no, udder. Rau clone Dolly, cov kws tshawb fawb thawj zaug tshem tawm cov nucleus los ntawm somatic cell ntawm cov pab yaj.
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