Cov txheej txheem:

Yuav ua li cas ua kom cov dej tsis muaj nuclease?
Yuav ua li cas ua kom cov dej tsis muaj nuclease?

Video: Yuav ua li cas ua kom cov dej tsis muaj nuclease?

Video: Yuav ua li cas ua kom cov dej tsis muaj nuclease?
Video: Tsis Muaj Peev Xwm Yuav Ua Li Cas 2024, Kaum ib hlis
Anonim

Nrov lus teb (1)

Ntxiv 0.1% DEPC rau MilliQ lossis Ob Chav Distilled dej - cia nws zaum hmo ntuj ntawm 37degC thiab tom qab ntawd Autoclave nws. Ua paub tseeb tias cov iav siv los kuj tau muab yaug nrog tib yam dej los yog kho nrog Chloroform los yog Ci hauv qhov cub cua kub (260degC) rau 4 teev. Nws yuav tsum tau npaj rau kev siv - ob qho tib si DNase thiab RNase dawb.

Kuj nug, ua li cas dej tsis muaj nuclease?

Nuclease - Dej Dawb yog npaj nyob rau hauv ib tug proprietary txheej txheem, uas yields DNase , RNase , thiab nuclease - dawb , deionized dej tsis siv tshuaj ntxiv, xws li diethylpyrocarbonate (DEPC). Nuclease - Dej Dawb yog muab rau hauv nuclease - dawb thawv.

Tsis tas li ntawd, puas yog Milliq dej nuclease dawb? Txij li thaum lub cartridge yog tsim los txuas nrog lub qhov hluav taws xob ntawm a dej purification system, xws li a Milli-Q ®, ib qho tuaj yeem tau txais ultrapure dej uas yog thiab dawb ntawm nucleases.

Tsis tas li ntawd, cov dej tsis muaj nuclease ua dab tsi?

Nuclease - dej dawb yog zoo tagnrho rau kev npaj cov reagents thiab siv rau hauv cov tshuaj tiv thaiv enzymatic. Tsis muaj tshuaj lom, xws li DEPC, yog siv rau hauv kev tsim cov khoom no dej , yog li kom tsis txhob muaj kev cuam tshuam hauv cov tshuaj tiv thaiv enzymatic.

Yuav ua li cas ua Depc dej?

DEPC Daim Ntawv Qhia Dej Kho Dej

  1. Ntxiv 1ml ntawm 0.1% Diethylpyrocarbonate (DEPC) rau 1000ml distilled dej.
  2. Sib tov zoo thiab cia nyob rau hauv chav tsev kub rau 1 teev.
  3. Autoclave.
  4. Cia txias rau chav sov ua ntej siv.

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