Cov txheej txheem:
Video: Yuav ua li cas ua kom cov dej tsis muaj nuclease?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nrov lus teb (1)
Ntxiv 0.1% DEPC rau MilliQ lossis Ob Chav Distilled dej - cia nws zaum hmo ntuj ntawm 37degC thiab tom qab ntawd Autoclave nws. Ua paub tseeb tias cov iav siv los kuj tau muab yaug nrog tib yam dej los yog kho nrog Chloroform los yog Ci hauv qhov cub cua kub (260degC) rau 4 teev. Nws yuav tsum tau npaj rau kev siv - ob qho tib si DNase thiab RNase dawb.
Kuj nug, ua li cas dej tsis muaj nuclease?
Nuclease - Dej Dawb yog npaj nyob rau hauv ib tug proprietary txheej txheem, uas yields DNase , RNase , thiab nuclease - dawb , deionized dej tsis siv tshuaj ntxiv, xws li diethylpyrocarbonate (DEPC). Nuclease - Dej Dawb yog muab rau hauv nuclease - dawb thawv.
Tsis tas li ntawd, puas yog Milliq dej nuclease dawb? Txij li thaum lub cartridge yog tsim los txuas nrog lub qhov hluav taws xob ntawm a dej purification system, xws li a Milli-Q ®, ib qho tuaj yeem tau txais ultrapure dej uas yog thiab dawb ntawm nucleases.
Tsis tas li ntawd, cov dej tsis muaj nuclease ua dab tsi?
Nuclease - dej dawb yog zoo tagnrho rau kev npaj cov reagents thiab siv rau hauv cov tshuaj tiv thaiv enzymatic. Tsis muaj tshuaj lom, xws li DEPC, yog siv rau hauv kev tsim cov khoom no dej , yog li kom tsis txhob muaj kev cuam tshuam hauv cov tshuaj tiv thaiv enzymatic.
Yuav ua li cas ua Depc dej?
DEPC Daim Ntawv Qhia Dej Kho Dej
- Ntxiv 1ml ntawm 0.1% Diethylpyrocarbonate (DEPC) rau 1000ml distilled dej.
- Sib tov zoo thiab cia nyob rau hauv chav tsev kub rau 1 teev.
- Autoclave.
- Cia txias rau chav sov ua ntej siv.
Pom zoo:
Cov hlwb twg tsis muaj nucleus thiab tsis muaj chromosomes?
Ib lub cell uas tsis muaj nucleus yog ib lub cell prokaryotic. Nws tsuas muaj cov khoom siv caj ces (DNA) hauv nws tab sis tsis muaj daim nyias nyias nyias nyias
Lub ntiaj teb yuav hloov li cas yog tias cov nyhuv tsev cog khoom tsis muaj nyob rau hauv tag nrho cov lus nug?
A) Yog tsis muaj cov nyhuv tsev cog khoom, lub ntiaj teb yuav tawg tag nrho nws cov cua sov rau hauv qhov chaw. b) Txhua lub hnub ci hluav taws xob tuaj yeem nqus tau yam tsis muaj lub tsev cog khoom. C) Qhov tshwm sim ntawm tsis muaj cov nyhuv tsev cog khoom yuav yog lub ntiaj teb kub heev uas yeej tsis txias
Cov npe twg kom raug qhia cov subshell hauv kev txiav txim kom muaj zog?
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Yuav ua li cas yog qhov tshwm sim uas ib tug poj niam dig muag xim uas yuav ib tug txiv neej uas tsis pom kev zoo yuav muaj xim dig muag me nyuam?
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Dab tsi yog qhov tseem ceeb tshaj plaws uas yuav tsum muaj rau cov kua dej ntws hauv cov kav dej Dab tsi lwm yam cuam tshuam rau cov dej ntws?
Thaum ib qho kev quab yuam sab nraud yog exerted rau ntawm cov kua uas muaj, qhov tshwm sim siab yog kis sib npaug thoob plaws hauv cov kua. Yog li txhawm rau kom dej ntws, dej xav tau qhov sib txawv siab. Cov kav dej kuj tuaj yeem cuam tshuam los ntawm cov kua dej, cov yeeb nkab loj, qhov kub thiab txias (cov kav dej khov), cov kua dej ntom ntom