Video: Vim li cas nws thiaj tseem ceeb rau incubate TSI slant nrog lub hau xoob?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nws yog tseem ceeb khaws cov caps ploj ntawm TSI nruab nrab kom tso cai rau qhov sib txawv hauv pH kom pom. Vim tias cov piam thaj ntau ntau, cov kua qaub txaus yog tsim los ntawm fermentation hauv pob tw kom qis pH ntawm ob lub pob tw thiab cov pob tw. slant , tig daj.
Tsis tas li ntawd, yog dab tsi yog ib tug TSI slant siv rau?
Triple qab zib hlau agar ( TSI ) yog qhov sib txawv nruab nrab uas muaj lactose, sucrose, ib qho me me ntawm cov piam thaj (dextrose), ferrous sulfate, thiab pH qhia phenol liab. Nws yog siv rau sib txawv enterics raws li lub peev xwm los txo cov sulfur thiab ferment carbohydrates.
Tsis tas li ntawd, vim li cas thiaj muaj lactose thiab sucrose ntau dua hauv TSI Agar? Ntxiv ntawm sucrose in TSI Agar tso cai pom ua ntej ntawm cov kab mob coliform uas ferment sucrose ntau nrawm dua lactose . Ntxiv sucrose kuj pab tus kev txheeb xyuas cov kab mob gram-negative uas tuaj yeem ferment sucrose tab sis tsis yog lactose.
Hauv qhov no, lub hom phiaj ntawm thiosulfate hauv TSI Agar yog dab tsi?
Sodium thiosulfate ua raws li lub substrate rau enzymatic txo thiab cov resultant colorless hydrogen sulfide gas reacts nrog ferrous sulfate los tsim ferrous sulfide, ib tug insoluble dub precipitate.
Lub hauv paus ntsiab lus ntawm TSI yog dab tsi?
Txoj Cai : Triple qab zib hlau agar test yog siv los txiav txim seb puas gram tsis zoo bacilli siv cov piam thaj thiab lactose los yog sucrose fermentatively thiab tsim hydrogen sulfide (H2S). Nws muaj 10 feem ntawm lactose: 10 feem ntawm sucrose: 1 feem ntawm qabzib thiab peptone.
Pom zoo:
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