Video: Yuav ua li cas cov tshuaj intercalating ua rau kev hloov pauv?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Intercalating agents , xws li ethidium bromide thiab proflavine, yog molecules uas tuaj yeem nkag ntawm cov hauv paus hauv DNA, ua rau frameshift kev hloov pauv thaum replication. Qee yam xws li daunorubicin tuaj yeem thaiv cov ntawv sau thiab rov ua dua, ua rau lawv muaj kuab lom heev rau proliferating cell.
Tsis tas li ntawd, yuav ua li cas cov tshuaj mutagenic ua rau kev hloov pauv?
Ua rau ntawm Kev hloov pauv Mutagens yog tej tus neeg sawv cev uas yuav induce kev hloov pauv . Cov no tus neeg sawv cev tej zaum yuav muaj tshuaj lom neeg kev hloov pauv los yog hluav taws xob, xws li lub teeb ultraviolet. Qee yam tshuaj mutagens yog lub hauv paus analogs thiab tau hloov mus rau hauv DNA thaum rov ua dua. Radiation kuj ua rau mutations.
Ib yam li ntawd, EtBr ua rau kev hloov pauv li cas? Raws li koj hais EtBr yog tshuaj mutagen ua tus neeg sawv cev intercalating. Cov intercalating agents no yog cov molecules tiaj tus uas tuaj yeem plam ntawm lub hauv paus khub hauv ob lub helix, me ntsis unwinding lub helix thiab li no nce qhov kev ncua deb ntawm cov khub uas nyob ib sab. Ua li no cov molecules ua rau tus kev hloov pauv.
Ib yam li ntawd, dab tsi yog induced mutations los ntawm?
Induced mutations tshwm sim tom qab kev kho mob ntawm lub cev nrog ib tug exogenous mutagen ua lub cev lossis tshuaj lom neeg ua rau muaj kev hloov pauv ntau zaus. Cov kab mob yog cov qauv yooj yim thiab dav siv rau kev kuaj mutagenesis thiab DNA kho cov txheej txheem.
Yuav ua li cas lub hauv paus analogue qhia txog kev hloov pauv?
Base analogue mutagens yog tshuaj lom neeg uas ua rau hauv paus mus zoo li lawv ua tau muab tso rau hauv DNA nyob rau hauv qhov chaw ntawm ib qho ntawm qhov qub hauv paus tab sis hauv ua yog li ua rau kom nce tus nqi ntawm kev hloov pauv . Yuav kom mutagenic, a puag analog yuav tsum mispair ntau zaus tshaj qhov qub puag nws hloov.
Pom zoo:
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Txij li thaum lub kaw lus raug cais tawm, tsis muaj cua sov tuaj yeem khiav tawm (tus txheej txheem yog li adiabatic), yog li thaum lub zog ntws tawm hauv lub cev, qhov entropy ntawm qhov system nce, piv txwv li Δ Ssys> 0. Yog li ntawd, lub entropy ntawm lub system yuav tsum tau nce rau ib tug spontaneous txheej txheem nyob rau hauv no cais system