Video: Koj tuaj yeem qhia TLC phaj li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
VIDEO
Ntawm no, TLC phaj yog dab tsi?
Nyias-txheej chromatography ( TLC ) yog cov txheej txheem chromatography siv los cais cov khoom sib xyaw uas tsis yog-volatile. Tom qab tus qauv tau siv rau ntawm lub phaj , ib qho hnyav lossis hnyav sib tov (paub tias yog theem mobile) yog kos los ntawm phaj los ntawm kev ua haujlwm capillary.
Tom qab ntawd, lo lus nug yog, koj yuav ua li cas rau TLC phaj? A TLC phaj tuaj yeem yog tsim tawm nyob rau hauv ib tug beaker los yog kaw lub thawv (saib daim duab hauv qab no). Muab ib qho me me ntawm cov kuab tshuaj (= theem mobile) rau hauv lub thawv. Cov qib hnyav yuav tsum nyob hauv qab kab pib ntawm cov TLC , txwv tsis pub cov pob yuav yaj mus. Sab ntug ntawm lub phaj yog ces dipped nyob rau hauv ib tug hnyav.
Kuj tseem paub yog, koj ua li cas TLC xeem?
Txiav lub TLC daim ntawv rau hauv strips kwv yees li 2 cm x 7 cm. Kos ib kab xaum rau ntawm sab luv, kwv yees li 0.5 cm ntawm hauv qab. Tsis txhob siv tus cwj mem raws li tus cwj mem yuav yaj hauv cov kuab tshuaj organic thiab cais tawm, ua rau tsis pom lossis ua paug rau koj cov txiaj ntsig. Ncuav cov kuab tshuaj los ua kuaj rau hauv lub khob ntim.
Yuav ua li cas yog tias koj siv ntau dhau ntawm TLC phaj?
Teb: Tus number case yuav mus nrog cov kuab tshuaj eluting thiab cais rau hauv nws cov pigments, muab koj ntau qhov chaw extraneous. 3) Yuav ua li cas tshwm sim yog koj chaw ntau dhau ntawm a compound ntawm TLC phaj ? Teb: Qhov chaw yuav qhia trailing. Teb: Cov pob yuav yaj mus rau hauv lub reservoir ntawm eluting hnyav.
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