Video: KM yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov units ntawm Km yog cov concentration piv txwv li mM, mM los yog Km yog cov concentration ntawm substrate uas ib nrab maximal tshaj tawm tau pom. Vmax tuaj yeem nthuav tawm ntau yam nyob ntawm seb cov ntaub ntawv twg muaj.
Tom qab ntawd, ib tug kuj yuav nug, dab tsi yog units rau Michaelis tas li KM?
Km me me qhia tias muaj kev sib raug zoo vim nws txhais tau tias cov tshuaj tiv thaiv tuaj yeem ncav cuag ib nrab ntawm Vmax nyob rau hauv ib tug me me ntawm substrate concentration. Tus me Km no yuav mus kom ze Vmax ceev tshaj tus nqi siab Km. Thaum Kcat / Km, nws muab peb ntsuas ntawm enzyme efficiency nrog ib chav tsev ntawm 1 / (Molarity * thib ob) = L / (mol * s).
Km thiab Vmax yog dab tsi? Tus nqi ntawm cov tshuaj tiv thaiv thaum lub enzyme saturated nrog substrate yog qhov siab tshaj plaws ntawm cov tshuaj tiv thaiv, Vmax . Qhov no feem ntau yog qhia raws li tus Km (Michaelis tas li) ntawm enzyme, ib qho kev ntsuas kev sib hloov ntawm kev sib raug zoo. Rau lub hom phiaj siv tau, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax.
Ib yam li ib tug yuav nug, dab tsi yog chav tsev ntawm Vmax?
Vmax "sawv cev tus nqi siab tshaj plaws ua tiav los ntawm qhov system, ntawm qhov siab tshaj plaws (saturating) substrate concentrations" (wikipedia). Chav tsev : umol/min (los yog mol/s). Tab sis tom qab ntawd cov kev ua enzymes ntawm cov qauv yog cov enzyme uas hloov 1 umole ntawm substrate / min nyob rau hauv qhov kev pom zoo.
Koj daws li cas rau KM?
Txhais Km siv qhov sib npaug Km -1 = k1/ (k-1 + k2). daws rau [ES] siv qhov sib npaug [ES] = [S][Et]/( Km + [S]) kom tau [Ef] thiab [S]. Siv [Ef] los txhais Vmax siv qhov sib npaug vmax = kcat [Et].
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