Puas yog ethanol nag lossis daus tshem tawm cov protein?
Puas yog ethanol nag lossis daus tshem tawm cov protein?

Video: Puas yog ethanol nag lossis daus tshem tawm cov protein?

Video: Puas yog ethanol nag lossis daus tshem tawm cov protein?
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Anonim

Phenol chloroform extraction (saib Kirby, 1957), ib txwm ua raws li ethanol nag lossis daus , yog txoj kev cai rau tshem tawm cov protein los ntawm a DNA qauv. Cov dej-soluble DNA partitions nyob rau hauv lub aqueous theem, thaum lub cov proteins denature nyob rau hauv lub xub ntiag ntawm organic solvents, yog li nyob rau hauv lub organic theem.

Ib yam li ntawd, puas ethanol precipitate protein?

Ethanol nag lossis daus yog ib txoj hauv kev tshem SDS thiab lwm yam cawv soluble impurities los ntawm protein ntau cov qauv nrog tsawg heev protein ntau poob. Daim ntawv qhia no tshawb xyuas protein ntau rov qab los ethanol nag lossis daus zoo li cov solubility SDS nyob rau hauv txias 90% ethanol.

Tom qab, lo lus nug yog, koj ua li cas ethanol nag lossis daus? Cov txheej txheem hauv paus yog tias ntsev thiab ethanol yog ntxiv rau cov tshuaj aqueous, uas yuam cov nag lossis daus ntawm nucleic acids tawm ntawm kev daws. Tom qab nag lossis daus cov nucleic acids ua tau tom qab ntawd muab cais tawm ntawm qhov seem ntawm kev daws los ntawm centrifugation.

Tib neeg kuj nug, puas yog ethanol nag lossis daus tshem tawm cov nucleotides dawb?

Phenol-chloroform Extraction thiab Ethanol nag lossis daus Rau tshem tawm ntawm cov protein thiab feem ntau dawb nucleotides , phenol: chloroform extraction thiab ethanol nag lossis daus ntawm RNA transcripts yog txoj kev nyiam.

RNA tshem tawm ntawm DNA li cas?

RNA Kev sib kis tuaj yeem raug tshem tawm los ntawm kev ntxiv 2 microlitre ntawm RNase A (10 mg / ml, Fermentas) mus rau 20 microlitre ntawm DNA yaj nyob rau hauv TE tsis (Tris–EDTA, pH = 8.0) thiab incubate rau 3-4 h ntawm 37 C.

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