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Video: Koj xam Km thiab Vmax li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Km and Vmax yog txiav txim los ntawm incubating lub enzyme nrog sib txawv concentrations ntawm substrate; Cov txiaj ntsig tuaj yeem npaj ua ib daim duab ntawm tus nqi ntawm cov tshuaj tiv thaiv (v) tiv thaiv cov ntsiab lus ntawm substrate ([S], thiab feem ntau yuav ua rau muaj qhov nkhaus hyperbolic, raws li qhia hauv cov duab saum toj no.
Tsis tas li ntawd, koj yuav suav km li cas?
Koj tuaj yeem kwv yees KM thiab Vmax los ntawm daim duab ntawm qhov pib tshaj tawm piv rau [S]
- Khiav cov kev cuam tshuam nrog tas li [Etot], sib txawv [S], thiab ntsuas Vo.
- Graph Vo vs. [S].
- Kwv yees Vmax los ntawm asymptote.
- Calculate Vmax/2.
- nyeem KM ntawm daim duab.
Kuj Paub, Km thiab Vmax yog dab tsi? Cov ib km yog cov concentration i.e. mM, mM los yog Km yog qhov concentration ntawm substrate uas ib nrab maximal tshaj tawm tau pom. Vmax tuaj yeem qhia ntau yam units nyob ntawm seb cov ntaub ntawv twg muaj.
Tsis tas li ntawd, chav tsev ntawm Vmax yog dab tsi?
Vmax "sawv cev tus nqi siab tshaj plaws ua tiav los ntawm qhov system, ntawm qhov siab tshaj plaws (saturating) substrate concentrations" (wikipedia). Chav tsev : umol/min (los yog mol/s). Tab sis tom qab ntawd cov kev ua enzymes ntawm cov qauv yog cov enzyme uas hloov 1 umole ntawm substrate / min nyob rau hauv qhov kev pom zoo.
Tus nqi Km yog dab tsi?
Michaelis tsis tu ncua ( KM ) yog txhais raws li lub substrate concentration uas cov tshuaj tiv thaiv tus nqi yog ib nrab ntawm nws maximal tus nqi (los yog hauv lwm lo lus nws txhais tau hais tias lub substrate concentration ntawm ib nrab ntawm cov chaw ua haujlwm tau nyob).
Pom zoo:
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Qhov no feem ntau yog qhia raws li Km (Michaelis tas li) ntawm cov enzyme, ib qho kev ntsuas qhov sib txawv ntawm affinity. Rau cov laj thawj, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax. plotting v tawm tsam v / [S] muab ib txoj kab ncaj nraim: y intercept = Vmax. gradient = -Km. x intercept = Vmax / Km