Cov txheej txheem:

Yuav ua li cas koj xam KMA ntawm Km thiab Vmax?
Yuav ua li cas koj xam KMA ntawm Km thiab Vmax?

Video: Yuav ua li cas koj xam KMA ntawm Km thiab Vmax?

Video: Yuav ua li cas koj xam KMA ntawm Km thiab Vmax?
Video: By:เสี่ยหนู koj puas tseem nco,sib hlub tsis pom txoj hmoo,thov bloke koj lawm [DJ Mey Ka] Remix 2024, Kaum ib hlis
Anonim

Qhov no feem ntau yog qhia raws li tus Km (Michaelis tas li) ntawm cov enzyme, ib qho inverse ntsuas ntawm affinity. Rau kev siv, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax.

plotting v tawm tsam v / [S] muab ib txoj kab ncaj nraim:

  1. y intercept = Vmax .
  2. gradient = - Km .
  3. x intercept = Vmax / Km .

Ib sab ntawm no, Michaelis Menten suav km li cas?

Cov kev sib npaug uas txhais cov Michaelis - Menten plot is: V = (Vmax [S]) ÷ (KM + [S}). Thaum lub sij hawm uas KM = [S], this kev sib npaug txo rau V = Vmax ÷ 2, KM yog sib npaug rau cov concentration ntawm cov substrate thaum lub tshaj tawm yog ib nrab ntawm nws cov nqi siab tshaj plaws.

Ib yam li ntawd, cov units rau Km thiab Vmax yog dab tsi? Cov units ntawm Km yog cov concentration i.e. mM, mM los yog Km yog qhov concentration ntawm substrate uas ib nrab maximal tshaj tawm tau pom. Vmax tuaj yeem hais tau ntau yam units nyob ntawm seb cov ntaub ntawv twg muaj.

Raws li, koj yuav suav km li cas?

Koj tuaj yeem kwv yees KM thiab Vmax los ntawm daim duab ntawm qhov pib tshaj tawm piv rau [S]

  1. Khiav cov kev cuam tshuam nrog tas li [Etot], sib txawv [S], thiab ntsuas Vo.
  2. Graph Vo vs. [S].
  3. Kwv yees Vmax los ntawm asymptote.
  4. Calculate Vmax/2.
  5. nyeem KM ntawm daim duab.

Tus nqi Km yog dab tsi?

Michaelis tsis tu ncua ( KM ) yog txhais raws li lub substrate concentration uas cov tshuaj tiv thaiv tus nqi yog ib nrab ntawm nws maximal tus nqi (los yog hauv lwm lo lus nws txhais tau hais tias lub substrate concentration ntawm ib nrab ntawm cov chaw ua haujlwm tau nyob).

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