Cov txheej txheem:
Video: Yuav ua li cas koj xam KMA ntawm Km thiab Vmax?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Qhov no feem ntau yog qhia raws li tus Km (Michaelis tas li) ntawm cov enzyme, ib qho inverse ntsuas ntawm affinity. Rau kev siv, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax.
plotting v tawm tsam v / [S] muab ib txoj kab ncaj nraim:
- y intercept = Vmax .
- gradient = - Km .
- x intercept = Vmax / Km .
Ib sab ntawm no, Michaelis Menten suav km li cas?
Cov kev sib npaug uas txhais cov Michaelis - Menten plot is: V = (Vmax [S]) ÷ (KM + [S}). Thaum lub sij hawm uas KM = [S], this kev sib npaug txo rau V = Vmax ÷ 2, KM yog sib npaug rau cov concentration ntawm cov substrate thaum lub tshaj tawm yog ib nrab ntawm nws cov nqi siab tshaj plaws.
Ib yam li ntawd, cov units rau Km thiab Vmax yog dab tsi? Cov units ntawm Km yog cov concentration i.e. mM, mM los yog Km yog qhov concentration ntawm substrate uas ib nrab maximal tshaj tawm tau pom. Vmax tuaj yeem hais tau ntau yam units nyob ntawm seb cov ntaub ntawv twg muaj.
Raws li, koj yuav suav km li cas?
Koj tuaj yeem kwv yees KM thiab Vmax los ntawm daim duab ntawm qhov pib tshaj tawm piv rau [S]
- Khiav cov kev cuam tshuam nrog tas li [Etot], sib txawv [S], thiab ntsuas Vo.
- Graph Vo vs. [S].
- Kwv yees Vmax los ntawm asymptote.
- Calculate Vmax/2.
- nyeem KM ntawm daim duab.
Tus nqi Km yog dab tsi?
Michaelis tsis tu ncua ( KM ) yog txhais raws li lub substrate concentration uas cov tshuaj tiv thaiv tus nqi yog ib nrab ntawm nws maximal tus nqi (los yog hauv lwm lo lus nws txhais tau hais tias lub substrate concentration ntawm ib nrab ntawm cov chaw ua haujlwm tau nyob).
Pom zoo:
Yuav ua li cas koj xam qhov zaus ntawm zaus thiab feem pua?
Ua li no, faib cov zaus los ntawm tag nrho cov txiaj ntsig thiab muab faib los ntawm 100. Hauv qhov no, qhov zaus ntawm thawj kab yog 1 thiab tag nrho cov txiaj ntsig yog 10. Qhov feem pua yog tom qab ntawd yog 10.0. Cov kab kawg yog qhov feem pua Cumulative
Yuav ua li cas koj xam ID los ntawm OD thiab phab ntsa thickness?
Yuav ua li cas xam Phab Ntsa Loj Raws li OD thiab ID Tshem tawm sab hauv txoj kab uas hla ntawm sab nraud ntawm cov yeeb nkab. Qhov tshwm sim yog qhov sib xyaw tuab ntawm cov yeeb nkab phab ntsa ntawm ob sab ntawm cov yeeb nkab. Faib tag nrho cov yeeb nkab phab ntsa thickness los ntawm ob. Qhov tshwm sim yog qhov loj, lossis tuab, ntawm ib lub yeeb nkab phab ntsa. Tshawb xyuas qhov yuam kev los ntawm kev thim rov qab cov kev suav
Yuav ua li cas koj xam amps ntawm volts thiab kuj?
Ohm txoj cai formula Lub resistor tam sim no kuv nyob rau hauv amps (A) yog sib npaug rau cov resistor voltage V nyob rau hauv volts (V) muab faib los ntawm cov kuj R nyob rau hauv ohms (Ω): V yog qhov voltage poob ntawm tus resistor, ntsuas nyob rau hauv Volts (V )
Yuav ua li cas koj xam qhov hnyav thiab qhov sib npaug ntawm lub dav hlau?
Ntxiv tag nrho cov sijhawm mus nrhiav lub sijhawm tag nrho. Faib tag nrho lub sijhawm los ntawm qhov hnyav tag nrho kom pom qhov nruab nrab ntawm lub ntiajteb txawj nqus. Nrhiav tag nrho qhov hnyav thiab qhov nruab nrab ntawm lub ntiajteb txawj nqus ntawm qhov nruab nrab ntawm lub ntiajteb txawj nqus daim ntawv qhia hauv koj lub dav hlau POH los txiav txim seb lub dav hlau puas nyob hauv qhov txwv
Koj xam Km thiab Vmax li cas?
Km thiab Vmax yog txiav txim los ntawm incubating lub enzyme nrog sib txawv concentrations ntawm substrate; Cov txiaj ntsig tuaj yeem raug teeb tsa raws li daim duab ntawm cov tshuaj tiv thaiv (v) tiv thaiv cov ntsiab lus ntawm substrate ([S], thiab feem ntau yuav ua rau muaj qhov nkhaus hyperbolic, raws li qhia hauv cov duab saum toj no