Electropherogram qhia dab tsi?
Electropherogram qhia dab tsi?

Video: Electropherogram qhia dab tsi?

Video: Electropherogram qhia dab tsi?
Video: Leng Yang - Yog Vim Dab Tsi (Official Full Song | Nkauj Tawm Tshiab) 2020/05/24 2024, Kaum ib hlis
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Nyob rau hauv lub teb ntawm noob caj noob ces, ib electropherogram yog ib zajlus ntawm DNA fragment qhov ntau thiab tsawg, feem ntau yog siv rau genotyping xws li DNA sequencing. Xws li electropherograms tej zaum yuav raug siv los txiav txim DNA kab ke genotypes, lossis genotypes uas yog raws li qhov ntev ntawm cov DNA tshwj xeeb.

Tom qab ntawd, ib tug kuj yuav nug, yuav ua li cas yog cov chromatograms Electropherograms generated?

A chromatogram (qee zaum kuj hu ua electropherogram ) yog qhov pom kev sawv cev ntawm DNA qauv ua los ntawm lub tshuab ua ntu zus (xws li Applied Biosystems ABI PRISM 7700 Sequence Detection System). Cov kab ntsuab saum toj no chromatogram peaks cov qhab nias siab.

Ib sab saum toj no, dab tsi yog qhov stutter peak? Stutter Peaks . Stutter peaks muaj nyob rau hauv yuav luag txhua txhua electropherogram. Stutter peaks me me ncov uas tshwm sim tam sim ua ntej lossis tom qab qhov tseeb ncov . Thaum lub sij hawm PCR amplification txheej txheem, cov polymerase yuav poob nws qhov chaw thaum luam ib tug strand ntawm DNA, feem ntau plam mus rau pem hauv ntej los yog rov qab plaub puag khub.

Tom qab ntawd, lo lus nug yog, lub luag haujlwm ntawm tus neeg ua tiav hauv cov txheej txheem ntawm DNA sequencing yog dab tsi?

A tus neeg ua tiav yog ib tug neeg uas nws txoj hauj lwm yog los soj ntsuam cov raw ib ntus cov ntaub ntawv nthuav tawm los ntawm electropherogram thiab tsim kom muaj qhov zoo ib ntus los ntawm kev kho thiab hu rau cov tshuaj tiv thaiv ntxiv los sau cov ntaub ntawv zoo dua qhov xav tau.

Koj tuaj yeem qhia tau li cas yog tias lub ncov hauv Electropherogram yog homozygous lossis heterozygous?

Cov mutant nucleotides yuav yog ib nrab ntawm qhov loj hauv kev siv ( ncov qhov siab) thiab yuav muaj xim ntxaib, yog nws yog heterozygous . Yog nws yog homozygous , cov ncov yuav yog ib xim rau uas nucleotide thiab yuav sib npaug qhov siab li ntawm nucleotides uas nyob ib sab.

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