Video: Electropherogram qhia dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nyob rau hauv lub teb ntawm noob caj noob ces, ib electropherogram yog ib zajlus ntawm DNA fragment qhov ntau thiab tsawg, feem ntau yog siv rau genotyping xws li DNA sequencing. Xws li electropherograms tej zaum yuav raug siv los txiav txim DNA kab ke genotypes, lossis genotypes uas yog raws li qhov ntev ntawm cov DNA tshwj xeeb.
Tom qab ntawd, ib tug kuj yuav nug, yuav ua li cas yog cov chromatograms Electropherograms generated?
A chromatogram (qee zaum kuj hu ua electropherogram ) yog qhov pom kev sawv cev ntawm DNA qauv ua los ntawm lub tshuab ua ntu zus (xws li Applied Biosystems ABI PRISM 7700 Sequence Detection System). Cov kab ntsuab saum toj no chromatogram peaks cov qhab nias siab.
Ib sab saum toj no, dab tsi yog qhov stutter peak? Stutter Peaks . Stutter peaks muaj nyob rau hauv yuav luag txhua txhua electropherogram. Stutter peaks me me ncov uas tshwm sim tam sim ua ntej lossis tom qab qhov tseeb ncov . Thaum lub sij hawm PCR amplification txheej txheem, cov polymerase yuav poob nws qhov chaw thaum luam ib tug strand ntawm DNA, feem ntau plam mus rau pem hauv ntej los yog rov qab plaub puag khub.
Tom qab ntawd, lo lus nug yog, lub luag haujlwm ntawm tus neeg ua tiav hauv cov txheej txheem ntawm DNA sequencing yog dab tsi?
A tus neeg ua tiav yog ib tug neeg uas nws txoj hauj lwm yog los soj ntsuam cov raw ib ntus cov ntaub ntawv nthuav tawm los ntawm electropherogram thiab tsim kom muaj qhov zoo ib ntus los ntawm kev kho thiab hu rau cov tshuaj tiv thaiv ntxiv los sau cov ntaub ntawv zoo dua qhov xav tau.
Koj tuaj yeem qhia tau li cas yog tias lub ncov hauv Electropherogram yog homozygous lossis heterozygous?
Cov mutant nucleotides yuav yog ib nrab ntawm qhov loj hauv kev siv ( ncov qhov siab) thiab yuav muaj xim ntxaib, yog nws yog heterozygous . Yog nws yog homozygous , cov ncov yuav yog ib xim rau uas nucleotide thiab yuav sib npaug qhov siab li ntawm nucleotides uas nyob ib sab.
Pom zoo:
Dab tsi yog qhov txawv ntawm daim ntawv qhia lub hom phiaj thiab daim ntawv qhia lub hom phiaj tshwj xeeb?
Qhov tseem ceeb hauv daim ntawv qhia dav dav yog nyob ntawm qhov chaw. Phab ntsa daim duab qhia chaw, feem ntau daim ntawv qhia pom nyob rau hauv atlases, thiab txoj kev maps yog tag nrho cov nyob rau hauv qeb no. Thematic maps, tseem hu ua daim ntawv qhia tshwj xeeb, qhia txog thaj chaw faib khoom ntawm ib lub ntsiab lus lossis qhov tshwm sim
Daim ntawv qhia dab tsi qhia txog qhov chaw ntawm thaj chaw?
Ntiaj Teb Kev Tshawb Fawb - Daim Ntawv Qhia Lub Ntiaj Teb Qhov Nto A B TOPOGRAPHIC MAP Daim duab qhia chaw uas qhia txog cov yam ntxwv ntawm thaj chaw. CONTOUR LINE Ib kab ntawm daim ntawv qhia saum toj kawg nkaus uas txuas cov ntsiab lus ntawm qhov siab sib npaug. CONTOUR INTERVAL Qhov txawv ntawm qhov nce ntawm ib kab contour mus rau lwm qhov
Kev taw qhia ntawm daim ntawv qhia yog dab tsi?
Kev taw qhia ntawm daim ntawv qhia yog kev sib raug zoo ntawm cov lus qhia ntawm daim ntawv qhia thiab cov lus qhia sib piv hauv kev muaj tiag. Lo lus 'orient' yog muab los ntawm Latin oriens, txhais tau tias sab hnub tuaj. Qhov feem ntau cartographic convention, yog tias sab qaum teb yog nyob rau sab saum toj ntawm daim ntawv qhia
Daim ntawv qhia lub cev ntawm Asmeskas qhia dab tsi?
Nqe lus piav qhia: Daim duab qhia lub cev ntawm Tebchaws Meskas qhia txog qhov nce siab, roob toj siab, toj siab, dej ntws, tiaj thiab lwm yam saum toj kawg nkaus ntawm Tebchaws Meskas. Tebchaws Meskas yog ib lub tebchaws loj uas muaj ntau yam ntawm lub cev, los ntawm cov roob siab mus rau qhov tob, hav dej, pas dej, thiab cov tiaj
Dab tsi yog zaj lus qhia ntawm Cyprus sim piav qhia los ntawm Mustapha Mond?
Dab tsi yog zaj lus qhia ntawm Cyprus sim piav qhia los ntawm Mustapha Mond? Ib haiv neeg ntawm Alphas tsis ua haujlwm. Lub neej ntawm Savage Reservation tuaj yeem rhuav tshem txhua qhov kev txias txias. Kev zoo siab yog tib txoj hauv kev rau kev vam meej ntawm tib neeg