Cov txheej txheem:

Yuav ua li cas cov kws tshawb fawb tsim cov recombinant DNA molecules?
Yuav ua li cas cov kws tshawb fawb tsim cov recombinant DNA molecules?

Video: Yuav ua li cas cov kws tshawb fawb tsim cov recombinant DNA molecules?

Video: Yuav ua li cas cov kws tshawb fawb tsim cov recombinant DNA molecules?
Video: Tshuaj theem vim li cas thiaj tau nqi ?/ Siv kho mob dab tsi?/paris polyphylla use for? 2024, Kaum ib hlis
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Cov txheej txheem ntawm Tsim kom muaj Recombinant DNA

Kev hloov pauv yog ib qho txheej txheem uas ib ntu ntawm DNA yog muab tso rau hauv lub plasmid - ib tug me me nws tus kheej-replicating lub voj voog ntawm DNA . Cov enzymes no yog tsim nyob rau hauv cov kab mob hlwb raws li ib tug tiv thaiv mechanism, thiab lawv tsom rau tej chaw ntawm a DNA molecule thiab txiav nws sib nrug.

Ib yam li ntawd, nws tau nug, yuav ua li cas thiaj tsim tau ib lub DNA molecule recombinant?

Tsim ntawm recombinant DNA yuav tsum muaj cloning vector, a DNA molecule uas replicates nyob rau hauv lub cell. Cov DNA ntu ua tau ua ke los ntawm kev siv ntau txoj hauv kev, xws li kev txwv enzyme / ligase cloning lossis Gibson sib dhos.

Yuav ua li cas recombinant DNA siv los tsim cov kab mob transgenic? A transgenic , los yog hloov caj ces , kab mob yog ib tug uas tau hloov los ntawm recombinant DNA technology, uas muaj xws li kev sib txuas ntawm DNA los ntawm cov genomes sib txawv los yog kev nkag ntawm txawv teb chaws DNA rau hauv genome.

Ib sab saum toj no, koj ua li cas tsim cov DNA plasmid recombinant?

Cov kauj ruam yooj yim yog:

  1. Txiav qhib lub plasmid thiab "muab tshuaj" rau hauv cov noob. Cov txheej txheem no tso siab rau kev txwv enzymes (uas txiav DNA) thiab DNA ligase (uas koom nrog DNA).
  2. Ntxig cov plasmid rau hauv cov kab mob.
  3. Loj hlob ntau cov kab mob plasmid-nqa thiab siv lawv ua "factories" los ua cov protein.

Yuav ua li cas yog ib co piv txwv ntawm recombinant DNA?

Los ntawm recombinant DNA Cov tswv yim, cov kab mob tau tsim muaj peev xwm ntawm kev sib txuas ntawm tib neeg cov tshuaj insulin, tib neeg kev loj hlob hormone, alpha interferon, tshuaj tiv thaiv kab mob siab B, thiab lwm yam khoom siv kho mob.

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