Tus nqi Km thiab Vmax txhais li cas?
Tus nqi Km thiab Vmax txhais li cas?

Video: Tus nqi Km thiab Vmax txhais li cas?

Video: Tus nqi Km thiab Vmax txhais li cas?
Video: yog yuav lwm tus ces mag xwb hhh 2024, Hlis ntuj nqeg
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Vmax yog sib npaug rau cov khoom ntawm cov catalyst tus nqi tas li (kcat) thiab cov concentration ntawm cov enzyme. Km yog qhov concentration ntawm substrates thaum cov tshuaj tiv thaiv mus txog ib nrab ntawm Vmax . Ib qho me me Km qhia siab affinity txij li nws txhais tau tias cov tshuaj tiv thaiv ua tau ncav cuag ib nrab ntawm Vmax nyob rau hauv ib tug me me ntawm substrate concentration.

Ib yam li ntawd, nws nug, Km thiab Vmax yog dab tsi?

Tus nqi ntawm cov tshuaj tiv thaiv thaum lub enzyme saturated nrog substrate yog qhov siab tshaj plaws ntawm cov tshuaj tiv thaiv, Vmax . Qhov no feem ntau yog qhia raws li tus Km (Michaelis tas li) ntawm enzyme, ib qho kev ntsuas kev sib hloov ntawm kev sib raug zoo. Rau lub hom phiaj siv tau, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax.

Kuj Paub, yog km nyob ntawm Vmax? feeb min Vmax nyob Nyob rau hauv tus txheej txheem ntawm enzyme nws tus kheej thiab cov concentration ntawm enzyme tam sim no. KM yog ib tug concentration substrate yuav tsum tau mus cuag lub siab tshaj plaws cov tshuaj tiv thaiv tshaj tawm - yog [S] >> Km ces Vo yuav nyob ze Vmax.

Ua raws li qhov xav tau, tus nqi Vmax txhais li cas?

Maximal tshaj tawm ntawm cov tshuaj tiv thaiv (lossis tus nqi siab tshaj) Vmax yog tus nqi attained thaum lub enzyme chaw yog saturated nrog substrate, piv txwv li thaum lub substrate concentration yog ntau dua li KM. Piv txwv li: Q8W1X2, Q9V2Z6. Cov Vmax nqi nyob ntawm tej yam kev mob, xws li pH, kub thiab ionic zog.

Qhov unit ntawm Vmax yog dab tsi?

Vmax "sawv cev tus nqi siab tshaj plaws ua tiav los ntawm qhov system, ntawm qhov siab tshaj plaws (saturating) substrate concentrations" (wikipedia). Chav tsev : umol/min (los yog mol/s). Thiab yog tias Vmax Nws yog nyob ntawm qhov concentration ntawm enzyme, tom kawg yuav tsum tau meej nrog rau lwm yam mob (pH, T °, )

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