Video: Dab tsi 2 dyes siv los ua cov khoom xyaw hauv EMB Agar?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Eosin methylene xiav ( EMB , tseem hu ua "Levine's formulation") yog ib qho kev xaiv rau cov kab mob Gram-negative. EMB muaj dyes uas yog tshuaj lom rau cov kab mob Gram-zoo. EMB yog qhov kev xaiv thiab sib txawv nruab nrab rau coliforms. Nws yog kev sib xyaw ntawm ob stains, eosin thiab methylene xiav hauv qhov piv ntawm 6: 1.
Tsis tas li ntawd, dab tsi dyes nyob rau hauv EMB Agar?
Eosin methylene blue agar (EMB) yog ib qho kev xaiv thiab sib txawv nruab nrab siv los cais cov quav coliforms. Eosin Y thiab methylene xiav yog pH qhov taw qhia dyes uas ua ke los ua ib qho tsaus liab ntshav precipitate ntawm pH tsawg; lawv kuj pab inhibit qhov kev loj hlob ntawm feem ntau Gram zoo kab mob.
Tom qab ntawd, lo lus nug yog, cov khoom xyaw tshwj xeeb ua rau MacConkey agar xaiv thiab cov khoom xyaw twg ua rau nws txawv? MacConkey Agar. Qhov no nruab nrab yog ob qho tib si xaiv thiab sib txawv. Cov khoom xyaw xaiv yog cov kua tsib ntsev thiab cov dye, siv lead ua violet uas inhibit qhov kev loj hlob ntawm cov kab mob Gram-zoo.
Tom qab ntawd, lo lus nug yog, dab tsi ua rau EMB xaiv thiab qhov sib txawv?
Eosin Methylene Xiav (los yog EMB ) Agar yog a Xaiv & Sib txawv Nruab nrab. Cov xaiv thiab sib txawv Qhov sib txawv yog vim cov xim Eosin Y thiab Methylene Blue, thiab cov suab thaj lactose thiab sucrose hauv nruab nrab. Nws yog Xaiv vim nws txhawb qee cov kab mob kom loj hlob thaum inhibiting lwm tus.
Puas E coli loj hlob ntawm EMB agar?
Tsuas yog cov kab mob gram-negative xwb loj hlob ntawm EMB agar . Cov kab mob Gram-zoo yog inhibited los ntawm dyes eosin thiab methylene xiav ntxiv rau agar . Vim muaj zog fermention ntawm lactose thiab tsim tawm ntau cov kua qaub, cov cheeb tsam ntawm Escherichia coli tshwm tsaus nti thiab xiav-dub nrog ib tug metallic ntsuab sheen.
Pom zoo:
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