Puas yog Cre lox thim rov qab?

Video: Puas yog Cre lox thim rov qab?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Txhua qhov kev sib koom ua ke sib kho los ntawm FLP lossis Cre yog thim rov qab . Thaum qhov kev txiav txim ntawm ib daim DNA flanked los ntawm loxP ib / FRT qhov chaw tau txais kev pom zoo tshaj nws qhov kev rov pib dua, kev hloov pauv thiab rov ua dua tshiab tshwm sim ntawm tib qhov tshwm sim. Cov loxP ib thiab FRT lub hom phiaj qhov chaw tau raug tsim kho kom tsis txhob muaj qhov teeb meem rov qab los.

Tom qab ntawd, CRE LOX ua haujlwm li cas?

Cre - Lox recombination koom nrog lub hom phiaj ntawm ib theem zuj zus ntawm DNA thiab splicing nws nrog kev pab los ntawm ib tug enzyme hu ua Cre recombinase . Cov Cre protein yog ib qhov chaw tshwj xeeb DNA recombinase uas tuaj yeem ua rau kev rov ua dua ntawm DNA ntawm cov chaw tshwj xeeb hauv DNA molecule.

Tsis tas li ntawd, Cre recombinase ua dab tsi? Cre recombinase yog ib tyrosine recombinase enzyme muab los ntawm P1 bacteriophage. Cov enzyme (38kDa) yog ib tug tswv cuab ntawm intgrase tsev neeg ntawm qhov chaw tshwj xeeb recombinase thiab nws yog paub los catalyze qhov chaw tshwj xeeb recombination tshwm sim ntawm ob qhov chaw DNA paub (LoxP sites).

Hais txog qhov no, tamoxifen induce Cre li cas?

Tamoxifen kev tswj hwm induces Cre recombinase qhia nyob rau hauv α-MHC txhawb nqa, zoo ib yam li cov nas saum toj no, uas ua rau lub plawv-cov leeg nqaij tshwj xeeb tshem tawm ntawm lub sijhawm tshwj xeeb.

LoxP qhov chaw yog dab tsi?

LoxP chaw yog cov kev taw qhia 34 bp ib ntus ua los ntawm ob qhov kev lees paub 13 bp qhov chaw cais los ntawm thaj tsam 8 bp spacer. Cov kab ke tsis tshwm sim nyob rau hauv ib qho kev paub genomes uas tsis yog P1 bacteriophage, thiab ntev txaus tias lawv tsis zoo li tshwm sim los ntawm lub sijhawm.

Pom zoo:

Vim li cas qee qhov kev hloov pauv tsis tuaj yeem thim rov qab?

Nws yuav ua tsis tau ntawv dua. Koj qhov siab yuav txo qis. Cov no yog cov kev hloov pauv tsis tau. Lawv tsis tuaj yeem thim rov qab txhua. Qhov sib txawv ntawm kev hloov pauv tsis tau thiab hloov tsis tau rov qab. Cov kev hloov pauv hloov tsis hloov Cov kev hloov pauv Feem ntau cov kev hloov ntawm lub cev yog hloov pauv tau. Tag nrho cov tshuaj hloov pauv yog hloov tsis tau

Koj ua li cas xam cov peev xwm thim rov qab?

Rau ib qho ion muab, qhov kev thim rov qab tuaj yeem suav tau los ntawm Nernst kab zauv qhov twg: R = gas tas li. T = kub (in oK) z = ion charge. Equilibrium (los yog thim rov qab) muaj peev xwm muaj peev xwm so ntawm -12 mV (raws li tsim los ntawm Na + / K + ATPase) tsis muaj voltage- lossis ligand-gated channels. thaum xub thawj, tsis muaj cov pa xau

Dab tsi yog qhov txawv ntawm kev ua txhaum thiab kev thim rov qab?

Kev hloov pauv yog kev hloov pauv hauv av ntawm ntug dej hiav txwv thaum rov qab los yog kev hloov pauv hauv hiav txwv. Cov ntsiab lus feem ntau yog siv rau kev hloov maj mam hloov ntawm ntug dej hiav txwv txoj hauj lwm yam tsis xav txog lub tshuab ua rau kev hloov pauv

Kev ua haujlwm ntawm HIV 1 thim rov qab transcriptase yog dab tsi?

Tus kab mob HIV-1 thim rov qab transcriptase enzyme yog lub luag haujlwm rau kev luam ib leeg-stranded viral RNA genome rau hauv ob-stranded DNA (Sarafianos et al, 2001). Cov DNA tsim tshiab tuaj yeem muab tso rau hauv tus tswv tsev genome; tus tswv tsev feem ntau yog tib neeg nyob rau hauv rooj plaub ntawm HIV