Lub luag haujlwm ntawm PCR tsis yog dab tsi?
Lub luag haujlwm ntawm PCR tsis yog dab tsi?

Video: Lub luag haujlwm ntawm PCR tsis yog dab tsi?

Video: Lub luag haujlwm ntawm PCR tsis yog dab tsi?
Video: Leng Yang - Yog Vim Dab Tsi (Official Full Song | Nkauj Tawm Tshiab) 2020/05/24 2024, Kaum ib hlis
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Keeb Kwm teb: Lub luag haujlwm yog dab tsi ntawm a tsis pub hauv a PCR ? Feem ntau, a tsis pub yog ib qho kev daws teeb meem uas tuaj yeem tiv taus cov kev hloov pauv pH los ntawm kev siv tshuaj nruab nrab me me ntawm cov tshuaj ntxiv acidic lossis cov ntsiab lus yooj yim, yog li tswj tag nrho pH ntawm nruab nrab. Vim li cas qhov no tsim nyog rau PCR ? DNA yog pH-sensitive.

Xav txog qhov no, dab tsi yog qhov tsis siv rau hauv PCR?

Tsis pub. PCR yog nqa tawm nyob rau hauv ib qho tsis muaj tshuaj lom neeg tsim nyog rau kev ua ntawm DNA polymerase. Qhov tsis pH feem ntau yog nyob nruab nrab ntawm 8.0 thiab 9.5 thiab feem ntau stabilized los ntawm Tris -HCl. Rau Taq DNA polymerase, ib qho kev tivthaiv nyob rau hauv qhov tsis yog poov tshuaj ion (K+) los ntawm KCl, uas txhawb nqa primer annealing.

Dab tsi yog qhov tsim nyog rau PCR? Basic Cheebtsam ntawm a PCR Cov tshuaj tiv thaiv muaj xws li DNA template, primers, nucleotides, DNA polymerase, thiab tsis.

Tsis tas li cov lus nug yog, qhov ua haujlwm ntawm Taq polymerase hauv PCR yog dab tsi?

“Qhov function of Taq DNA polymerase hauv PCR cov tshuaj tiv thaiv yog los nthuav cov DNA rau kev tsim ntau cov ntawv luam ntawm nws. Taq DNA polymerase yog DNA thermostable polymerase uas tuaj yeem ua haujlwm ntawm qhov kub siab dua."

Lub luag haujlwm ntawm MgCl2 hauv PCR yog dab tsi?

Lub luag haujlwm ntawm MgCl2 hauv PCR Kev tshwm sim. Cov Lub luag haujlwm ntawm MgCl2 hauv PCR cov tshuaj tiv thaiv yog los txhim kho DNA amplification los ntawm boosting cov kev ua ntawm Taq DNA polymerase. Cov DNA polymerase, dNTPs , primers thiab PCR tsis yog siv los ua raw khoom rau amplifying cov noob paj.

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