Video: Puas yog Michaelis Menten sib npaug siv rau tag nrho cov enzymes?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Tsis zoo li ntau cov enzymes , ua allosteric enzymes ua tsis mloog lus Michaelis - Menten kinetics. Yog li, allosteric cov enzymes qhia qhov nkhaus sigmodial qhia saum toj no. Lub ntsiab lus rau cov tshuaj tiv thaiv tshaj tawm, vo, piv rau substrate concentration ua tsis pom cov phiaj xwm hyperbolic kwv yees siv cov Michaelis - Menten equation.
Ntawm no, qhov sib npaug Michaelis Menten siv rau dab tsi?
Cov Michaelis - Menten equation (saib hauv qab) feem ntau yog siv rau kawm cov kinetics ntawm cov tshuaj tiv thaiv catalysis los ntawm enzymes thiab cov kinetics ntawm thauj los ntawm transporters. Feem ntau, tus nqi ntawm cov tshuaj tiv thaiv (los yog cov tshuaj tiv thaiv tshaj tawm) yog sim ntsuas ntawm ob peb lub substrate concentration qhov tseem ceeb.
Tsis tas li ntawd, puas yog Vmax thiab Km qhov tseem ceeb rau qhov muab enzyme? Tus nqi ntawm cov tshuaj tiv thaiv thaum lub enzyme yog saturated nrog substrate yog qhov siab tshaj plaws ntawm cov tshuaj tiv thaiv, Vmax . Qhov no feem ntau yog qhia raws li tus Km (Michaelis tas li ) ntawm enzyme , inverse ntsuas ntawm affinity. Rau kev siv, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax.
Hauv qhov no, Michaelis Menten enzymes yog dab tsi?
Cov Michaelis - Menten equation tshwm sim los ntawm kev sib npaug sib npaug rau ib enzymatic cov tshuaj tiv thaiv: E + S ↔ ES ↔ E + P, qhov twg E yog qhov enzyme , S yog lub substrate, ES yog tus enzyme -substrate complex, thiab P yog cov khoom. Yog li ntawd, ES complex yuav yaj rov qab rau hauv lub enzyme thiab substrate, los yog txav mus rau pem hauv ntej los tsim cov khoom.
Yuav ua li cas koj xam Michaelis tas li?
Cov kev sib npaug uas txhais cov Michaelis -Menten plot is: V = (Vmax [S]) ÷ (KM + [S}). Thaum lub sij hawm uas KM = [S], this kev sib npaug txo rau V = Vmax ÷ 2, KM yog sib npaug rau cov concentration ntawm cov substrate thaum lub tshaj tawm yog ib nrab ntawm nws cov nqi siab tshaj plaws.
Pom zoo:
Yuav daws qhov tsis sib npaug ntawm cov kab sib npaug thiab cov kab sib npaug sib npaug li cas?
Kev daws qhov tsis sib npaug ntawm qhov sib npaug yog zoo ib yam li kev daws cov kab sib npaug. Qhov sib txawv tseem ceeb yog koj tig lub cim tsis sib xws thaum faib lossis muab faib los ntawm tus lej tsis zoo. Graphing linear inequalities muaj ob peb qhov sib txawv ntxiv. Qhov uas yog qhov ntxoov ntxoo muaj xws li qhov tseem ceeb uas qhov kev tsis sib xws ntawm cov kab tawm muaj tseeb
Puas yog txhua qhov sib npaug sib npaug sib npaug?
Thawj qhov sib npaug sib npaug yog qhov tseeb yog tias nws muaj kev txuag kom muaj nuj nqis. Piv txwv li, qhov sib npaug sib cais yeej ib txwm muaj tseeb, txij li lub ntsiab lus lawv yog ntawm daim ntawv: M(y) + N(t) = 0, yog li ϕ(t, y) = A(y) + B(t) yog a khaws cia kom muaj nuj nqis
Dab tsi yog qhov sib npaug siv los xam tag nrho cov khoom siv hluav taws xob siv?
Cov mis uas txuas lub zog thiab lub zog yog: Zog = Lub Zog x Sijhawm. Chav tsev ntawm lub zog yog joule, lub zog ntawm lub zog yog watt, thiab chav tsev ntawm lub sijhawm yog qhov thib ob
Puas yog kev ua haujlwm sib npaug rau tag nrho lub zog?
Lub hauv paus ntsiab lus ntawm kev ua haujlwm thiab lub zog kinetic (tseem hu ua txoj haujlwm ua haujlwm-zog) hais tias txoj haujlwm ua los ntawm tag nrho cov zog ua haujlwm ntawm aparticle sib npaug ntawm qhov hloov pauv ntawm lub zog kinetic ntawm cov particle
Dab tsi yog qhov sib txawv ntawm cov kab lus sib npaug thiab qhov sib npaug sib npaug?
Cov kab lus sib npaug muaj qhov muaj txiaj ntsig zoo ib yam tab sis tau nthuav tawm nyob rau hauv ib hom ntawv sib txawv uas siv cov khoom ntawm cov lej xws li ax + bx = (a + b) x yog cov kab lus sib npaug. nruj me ntsis, lawv tsis yog 'sib npaug', yog li peb yuav tsum siv 3 kab sib luag hauv 'sib npaug' es tsis yog 2 raws li qhia ntawm no