Yuav ua li cas ua ammonium sulphate nag lossis daus?
Yuav ua li cas ua ammonium sulphate nag lossis daus?

Video: Yuav ua li cas ua ammonium sulphate nag lossis daus?

Video: Yuav ua li cas ua ammonium sulphate nag lossis daus?
Video: Koj mus ua neej zoo li cas lawm 11/16/2018 2024, Hlis ntuj nqeg
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Ntxiv cov khoom ammonium sulfate maj mam stirring; tso cai rau yaj ua ntej ntxiv cov khoom, sim tiv thaiv foaming. Online tshuab xam zauv ua tau nkag mus kom yooj yim txiav txim siab cov khoom ammonium sulfate yuav tsum tau mus cuag ib tug muab saturation.

Ntxiv rau qhov no, nag lossis daus ammonium sulfate ua haujlwm li cas?

Mechanism. Ammonium sulfate , nrog rau lwm cov ntsev nruab nrab, yuav stabilize cov proteins los ntawm kev nyiam kev daws teeb meem. Cov protein yog feem ntau khaws cia rau hauv ammonium sulfate vim nws inhibits cov kab mob loj hlob. Ntawm lub zog txaus ionic, cov protein yuav los nag tawm ntawm cov tshuaj, ib qho nyhuv hu ua "salting tawm".

Ib yam li ntawd, koj ua li cas yaj ammonium sulfate? Npaj saturated daws ntawm Ammonium sulfate , piv txwv li ~ 550g ua rau 1L distilled dej. Kub maj mam mus yaj tag nrho cov Ammonium sulfate thiab cia txias rau qhov kub thiab txias ua haujlwm ntawm lub tshuab magnetic stirrer. Crystals ntawm ammonium sulfate yuav tsum tsim thiab yuav qhia rau koj hais tias cov tshuaj yog tag nrho saturated.

Yog li ntawd, koj yuav ua li cas 100 Ammonium sulfate tov?

Rau ua 100 % saturated ammonium sulfate ntawm 25oC: Rau 1000 ml dH2O ntxiv 766.8 g ntawm (NH4)2YOG4. pH rau 6.8-7.0 nrog 5 los yog 10 M NaOH. Yog li, muaj 541.2 g ntawm ammonium sulfate rau 1 ntawm saturated daws.

Puas yog ammonium sulfate denature proteins?

Vim ammonium sulfate nag lossis daus tsuas yog txo cov solubility ntawm cov proteins thiab ua tsis yog denature lawv cov proteins tuaj yeem ua kom muaj zog los ntawm kev tshem tawm qhov seem ammonium sulfate daws ces cov protein ntau pellet tuaj yeem hloov kho hauv cov txheej txheem buffers los yog qis dua concentration ntawm ammonium sulfate.

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