Dab tsi yog lub luag haujlwm ntawm DNA ligase hauv DNA replication?
Dab tsi yog lub luag haujlwm ntawm DNA ligase hauv DNA replication?

Video: Dab tsi yog lub luag haujlwm ntawm DNA ligase hauv DNA replication?

Video: Dab tsi yog lub luag haujlwm ntawm DNA ligase hauv DNA replication?
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DNA ligase yog ib qho enzyme uas kho irregularities los yog tawg nyob rau hauv lub caj qaum ntawm ob-stranded DNA molecules. Nws muaj peb yam muaj nuj nqi : Nws seals kho nyob rau hauv lub DNA , nws seals recombination fragments, thiab nws txuas Okazaki fragments (me me DNA fragments tsim thaum lub sij hawm rov ua dua ntawm ob-stranded DNA ).

Ib yam li ntawd, nws tau nug, qhov ua haujlwm ntawm DNA ligase yog dab tsi?

DNA ligase yog ib hom enzyme, a ligase , (EC 6.5. 1.1) uas txhawb kev koom nrog DNA strands ua ke los ntawm catalyzing tsim ntawm phosphodiester daim ntawv cog lus.

DNA polymerase thiab DNA ligase ua haujlwm li cas hauv DNA replication? Thaum twg rov ua dua pib, ob niam txiv DNA strands raug cais. Okazaki fragments yog tsim los ntawm DNA polymerase ua hauj lwm rau luv luv nyob rau hauv lub 3' mus rau 5' kev taw qhia. Cov tawg yog khi ua ke los ntawm enzyme DNA ligase nyob rau hauv thiaj li yuav ua kom tiav rov ua dua nyob rau hauv lub lagging strand ntawm DNA.

Tsis tas li ntawd, lub luag haujlwm ntawm DNA polymerase hauv DNA replication yog dab tsi?

DNA polymerase yog ib qho enzyme uas synthesizes DNA molecules los ntawm deoxyribonucleotides, lub tsev blocks ntawm DNA . Cov enzymes no tseem ceeb rau DNA replication thiab feem ntau ua haujlwm ua khub los tsim ob qho tib si DNA strands los ntawm ib qho qub DNA molecule.

DNA ligase siv rau dab tsi?

DNA ligase yog a DNA - koom nrog enzyme. Yog ob daim DNA muaj qhov sib npaug, ligase tuaj yeem txuas lawv los ua ib qho, tsis muaj qhov sib txawv ntawm cov molecule DNA . Hauv DNA cloning, txwv enzymes thiab DNA ligase yog siv rau ntxig cov noob thiab lwm daim ntawm DNA hauv plasmids.

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