Video: Vim li cas salmonella siv hauv Ames kuaj?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Ames xeem tsim los ntawm tus kws tshawb fawb Bruce Ames ”yog siv txhawm rau ntsuam xyuas qhov muaj peev xwm carcinogenic ntawm cov tshuaj los ntawm kev siv cov kab mob kab mob Salmonella typhimurium. Cov kab no yog mutant rau biosynthesis ntawm histidine amino acid. Yog li ntawd lawv tsis tuaj yeem loj hlob thiab tsim cov cheeb tsam hauv nruab nrab tsis muaj histidine.
Hais txog qhov no, lub hom phiaj ntawm Ames xeem yog dab tsi?
Cov Ames xeem yog ib txoj hauv kev uas siv cov kab mob mus rau xeem seb ib qho tshuaj twg tuaj yeem ua rau muaj kev hloov pauv hauv DNA ntawm cov xeem kab mob. Nws yog ib qho tshuaj lom neeg soj ntsuam uas yog siv los soj ntsuam cov peev xwm mutagenic ntawm cov tshuaj sib txuas.
Kuj Paub, lub hom phiaj ntawm kev siv daim siab enzyme s9 extract hauv Ames kuaj yog dab tsi? Siv ntawm a daim siab homogenate simulates cov metabolic kev puas tsuaj ntawm qhov xav tias mutagen nyob rau hauv ib tug tsiaj lub cev, thiab ntau yog kwv yees mutagenicity ntawm cov khoom noj los ntawm tib neeg.
Ib sab saum toj no, cov kab mob twg siv hauv Ames kuaj?
Salmonella
Qhov kev xeem Ames yog dab tsi thiab nws ua haujlwm li cas?
Cov Ames xeem siv ntau hom kab mob sib txawv ntawm cov kab mob Salmonella los qhia pom muaj kev hloov pauv. Thaum ntxiv cov mutagens thiab daim siab enzymes, kev hloov pauv rov qab yuav tshwm sim thiab lawv yuav loj hlob tuaj.
Pom zoo:
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Cov lus nug nug tus neeg kuaj xyuas kom piav qhia tias vim li cas Ames kuaj rau mutagens tuaj yeem siv los kuaj cov carcinogens. Hauv kev xeem Ames, cov tshuaj uas ua rau muaj kev hloov pauv hauv Salmonella kuaj kab mob tuaj yeem yog cov carcinogens, vim tias lawv hloov pauv DNA thiab DNA hloov pauv tuaj yeem ua rau mob qog noj ntshav (B)
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Lub npe ntawm cov enzyme uas kuaj pom hauv qhov kev kuaj pom zoo catalase yog dab tsi?
Catalase Test- Ntsiab Cai, Siv, Txheej Txheem, Txhais Lus Txhais nrog ceev faj. Qhov kev sim no qhia pom tias muaj catalase, ib qho enzyme uas ua rau cov pa tawm ntawm cov pa oxygen los ntawm hydrogen peroxide (H2O2)
Yuav ua li cas cov khoom siv caj ces hauv txhua lub xov tooj tshiab tsim los ntawm kev faib tawm ntawm tes sib piv nrog cov khoom siv caj ces hauv lub xov tooj qub?
Mitosis tshwm sim nyob rau hauv ob lub nuclei uas zoo tib yam rau thawj nucleus. Yog li, ob lub hlwb tshiab tsim tom qab kev faib tawm ntawm tes muaj tib yam khoom siv caj ces. Thaum lub sij hawm mitosis, chromosomes condense los ntawm chromatin. Thaum saib nrog lub microscope, chromosomes pom nyob rau hauv lub nucleus