Video: RFLP tsim li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Hauv RFLP kev tsom xam, DNA qauv yog zom rau hauv cov khoom tawg los ntawm ib lossis ntau qhov kev txwv enzymes, thiab cov txiaj ntsig txwv tsis pub dhau los ntawm gel electrophoresis raws li lawv qhov loj me.
Tib neeg kuj nug, RFLP yog dab tsi lawv ua li cas?
Kev txwv Fragment Length Polymorphism ( RFLP ) Ib RFLP Kev sojntsuam yog ib daim ntawv sau npe DNA sib lawv liag nrog ib lossis ntau qhov seem ntawm cov qauv DNA zom tom qab lawv tau sib cais los ntawm gel electrophoresis, yog li nthuav tawm qhov tshwj xeeb blotting tus yam ntxwv rau ib qho genotype ntawm ib qho chaw tshwj xeeb.
Qhov thib ob, RFLP hauv biology yog dab tsi? Kev txwv tsis pub tawg ntev polymorphisms, lossis RFLPs , yog qhov sib txawv ntawm cov tib neeg nyob rau hauv qhov ntev ntawm DNA fragments txiav los ntawm enzymes. RFLP kev soj ntsuam tuaj yeem siv los ua ib hom kev kuaj caj ces los soj ntsuam seb tus neeg puas muaj cov noob caj noob ces rau tus kab mob uas khiav hauv nws tsev neeg.
Tsis tas li ntawd, thaum twg RFLP tau siv thawj zaug?
Kev txwv tsis pub tawg ntev polymorphism ( RFLP ) yog ib qho txheej txheem tsim nyob rau xyoo 1984 los ntawm tus kws tshawb fawb Askiv Alec Jeffreys thaum tshawb fawb txog cov kab mob keeb kwm.
Vim li cas RFLP tsis siv lawm?
Cov txheej txheem siv PCR amplification ntawm DNA nrog primers uas muaj fluorescent daim ntawv lo. RFLP tsom xam muaj tsis muaj lawm ua dav siv tab sis nws tseem yog ib qho tseem ceeb hauv kev tsim peb txoj kev nkag siab ntawm DNA tsom xam, thaum tseem txhawb kev txhim kho cov txheej txheem tshiab, muaj txiaj ntsig zoo dua.
Pom zoo:
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