Video: Lub hom phiaj ntawm Decolorizing nyob rau hauv tej yam txawv txawv yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nws yog siv los ua kom sib txawv ntawm grams cov kab mob zoo thiab grams cov kab mob tsis zoo. Li no, nws yog a sib txawv stain . Decolorizing lub cell ua rau cov phab ntsa tuab no kom lub cev qhuav dej thiab ntsws, uas kaw cov pores hauv phab ntsa ntawm tes thiab tiv thaiv cov stain los ntawm kev tawm ntawm lub cell.
Yog li ntawd, lub hom phiaj ntawm qhov sib txawv ntawm qhov stain yog dab tsi?
Differential Staining yog a staining txheej txheem uas siv ntau tshaj ib qho tshuaj stain . Kev siv ntau yam stains tuaj yeem sib txawv ntawm cov kab mob sib txawv lossis cov qauv / cellular Cheebtsam ntawm ib hom kab mob. Qhov kev sim no muaj txiaj ntsig zoo vim tias muaj ntau yam kab mob hloov pauv ntawm qee cov qe ntshav dawb.
Qhov thib ob, lub hom phiaj ntawm kev haus cawv hauv Gram stain yog dab tsi? Cov lub hom phiaj ntawm cawv hauv Gram stain yog pab lipids yaj hauv decolorizer (Morton, 2013, Pg. 31). Lawv kuj tau ntxuav tawm nrog crystal-violet iodine complex's. Lub txee stain uas siv yog hu ua: SaFranin thiab yog siv los ua tus counterstain rau Gram - kab mob tsis zoo.
Yog li ntawd, lub hom phiaj ntawm Decolorizer hauv Gram staining yog dab tsi?
A decolorizer xws li ethyl cawv los yog acetone ntxiv rau cov qauv, uas dehydrates peptidoglycan txheej, shrinking thiab ceev nws. Lub loj siv lead ua violet-iodine complex tsis muaj peev xwm nkag mus rau cov txheej txheem peptidoglycan nruj, thiab yog li daig hauv cell hauv Gram cov kab mob zoo.
Lub hom phiaj ntawm qhov counterstain hauv cov kua qaub ceev yog dab tsi?
Cov counterstain stains tsis- kua qaub - ceev cov kab mob xiav yog siv Methylene Blue los yog ntsuab yog siv Brilliant Green. Koj nyuam qhuav kawm 7 nqe lus!
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