Cov txheej txheem:

Northern blot test siv rau dab tsi?
Northern blot test siv rau dab tsi?

Video: Northern blot test siv rau dab tsi?

Video: Northern blot test siv rau dab tsi?
Video: Hunter Education with Kong Pheng Yang 3-25-2019 2024, Kaum ib hlis
Anonim

Cov qaum teb blot , los yog RNA blot , yog ib tug txheej txheem siv hauv kev tshawb fawb molecular biology los kawm txog noob caj noob ces los ntawm kev tshawb pom ntawm RNA (los yog cais mRNA) hauv ib qho qauv.

Ib yam li ib tug yuav nug, Northern blot ntes dab tsi?

A qaum teb blot yog ib txoj kev sim siv rau ntes tshwj xeeb RNA molecules ntawm kev sib xyaw ntawm RNA. Northern blotting tuaj yeem siv los txheeb xyuas tus qauv ntawm RNA los ntawm cov ntaub so ntswg lossis hom cell txhawm rau ntsuas RNA kev qhia ntawm cov noob tshwj xeeb.

Tom qab ntawd, lo lus nug yog, dab tsi yog Southern thiab Northern blotting? Southern thiab Northern blot hybridization. Southern blot hybridization yog hais txog kev tshawb nrhiav cov DNA tshwj xeeb uas tau muab cais los ntawm gel electrophoresis (Daim duab 1). Ib qho adaptation ntawm Southern blotting yog Northern blotting , nyob rau hauv uas RNA molecules yog electrophoresed los ntawm gel es tsis txhob ntawm DNA.

Tsis tas li ntawd, koj ua li cas Northern blot?

Cov kauj ruam koom nrog hauv Northern tsom xam suav nrog:

  1. Kev rho tawm RNA (tag nrho lossis poly (A) RNA)
  2. soj ntsuam tiam.
  3. Denaturing agarose gel electrophoresis.
  4. Hloov mus rau cov khoom txhawb nqa thiab immobilization.
  5. Prehybridization thiab hybridization nrog sojntsuam.
  6. Ntxuav.
  7. Kev kuaj pom.
  8. Stripping thiab reprobing (yeem)

Dab tsi yog Southern blot qhia koj?

Southern blotting yog cov txheej txheem kuaj siv los kuaj xyuas cov DNA tshwj xeeb hauv cov ntshav lossis cov ntaub so ntswg. Ib qho kev txwv enzyme yog siv los txiav cov qauv ntawm DNA rau hauv cov khoom tawg uas sib cais siv gel electrophoresis. Cov DNA tawg yog kis tawm ntawm cov gel mus rau saum npoo ntawm daim nyias nyias.

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