Video: Yuav ua li cas contigs assembled rau hauv scaffolds?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Thaum tsim cov qauv genome, tus neeg nyeem ntawm DNA yog thawj zaug assembled rau hauv contigs , uas, los ntawm qhov ntawm lawv kev sib dhos , muaj qhov sib txawv ntawm lawv. Cov kauj ruam tom ntej yog rau ces txuas qhov khoob ntawm no sib to tsim ib scaffold . Qhov no tuaj yeem ua tiav los ntawm kev siv optical mapping lossis mate-pair sequencing.
Ib yam li ntawd, koj tuaj yeem nug, dab tsi yog contigs thiab scaffolds?
A scaffold yog ib feem ntawm cov genome ib ntus reconstructed los ntawm kawg-sequenced tag nrho-genome shotgun clones. Scaffolds muaj li ntawm sib thiab qhov khoob. A sib yog qhov sib txuas ntev ntawm genomic ib ntus uas qhov kev txiav txim ntawm cov hauv paus paub txog qib kev ntseeg siab. Qee zaum, scaffolds tuaj yeem sib tshooj.
Tsis tas li ntawd, dab tsi yog contigs hauv bioinformatics? A sib (los ntawm contiguous) yog ib txheej ntawm overlapping DNA ntu uas ua ke sawv cev rau thaj tsam kev pom zoo ntawm DNA. Contigs Yog li tuaj yeem xa mus rau ob qho tib si sib tshooj DNA thiab sib tshooj ntawm lub cev (fragments) muaj nyob rau hauv clones nyob ntawm cov ntsiab lus.
Hauv qhov no, cov kab mob sib sau ua ke li cas?
Cov txheej txheem ntawm kev sib tshooj DNA ntawm DNA tawg yog hu ua a sib . Contig mapping yog ib txoj kev uas overlapping clones sib dhos mus ua ntu zus uas sib tshooj. Qhov no suav nrog kev teeb tsa sib nyob rau hauv kev txiav txim thiab orientation. Clone sib tuaj yeem ua tau sib dhos siv lawv cov BAC-kawg sequences.
Vim li cas cov kab mob tseem ceeb?
Contig assembly yog ib tseem ceeb kauj ruam hauv genome los ua ke. Rau daim ntawv qhia, overlapping clones tau sib sau ua ke kom sib tshooj. Txhua feem yog cloned nyob rau hauv ib tug vector thiab sequenced los ntawm ob qho tib si kawg los ua ib tug ib ntus ntev ntawm kwv yees li 600-700 bp. Qhov sib lawv liag ntawm ob qhov kawg ntawm DNA tawg yog hu ua khub kawg.
Pom zoo:
Yuav ua li cas cov txheej txheem ntawm cell faib nyob rau hauv eukaryotes feem ntau zoo ib yam li cell faib nyob rau hauv prokaryotes?
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Yuav ua li cas resistors coj nyob rau hauv series thiab nyob rau hauv parallel?
Txhua tus resistor nyob rau hauv ib tug series Circuit Court muaj tib tus nqi ntawm tam sim no ntws los ntawm nws. Txhua tus resistor nyob rau hauv ib qho hluav taws xob sib txuas muaj tib qhov hluav taws xob tag nrho ntawm qhov siv rau nws. Qhov tam sim no ntws los ntawm txhua tus resistor nyob rau hauv ib qho kev sib txuas sib txuas sib txawv, nyob ntawm qhov tsis kam
Yuav ua li cas cov xwm txheej yuav tsum tau induce ib tug tam sim no nyob rau hauv ib lub voj ntawm hlau?
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