Video: Dab tsi yog tus ntaiv hauv biology?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Teb: A “ ntaiv ua hauj lwm raws li ob qho tib si tswj thiab cov cuab yeej uas ntsuas qhov hnyav ntawm macromolecules zoo li DNA hauv gel electrophoresis. A ntaiv yog ib qho kev daws teeb meem uas muaj cov txheej txheem DNA uas tau hais kom zoo ntawm qhov ntev.
Ib yam li ntawd, tib neeg nug, dab tsi yog DNA ntaiv siv rau?
Qhov loj me me molecular tus cim , kuj hu ua protein ntaiv , DNA ntaiv , los yog RNA ntaiv , yog ib txheej txheem uas yog siv rau txheeb xyuas qhov kwv yees qhov loj me ntawm cov molecule khiav ntawm ib lub gel thaum lub sij hawm electrophoresis, siv lub hauv paus ntsiab lus uas molecular hnyav yog inversely proportional rau migration rate los ntawm ib tug gel.
Tsis tas li ntawd, 1 kb ntaiv txhais li cas? Cov 1 Kb DNA ntaiv yog ib qho kev sib xyaw tshwj xeeb ntawm ntau tus tswv plasmids zom nrog qhov tsim nyog txwv cov enzymes thiab cov khoom PCR kom tawm tau 13 feem, tsim nyog siv raws li cov qauv hnyav molecular rau electrophoresis.
Kuj nug, dab tsi yog tus ntaiv hauv PCR?
Txheej txheem cej luam. DNA ntaiv muaj cov txheej txheem DNA ntawm ntau qhov sib txawv. Cov DNA tawg no tau muab cais thiab pom zoo li DNA bands ntawm agarose lossis SDS DNA gels. DNA ntaiv yog siv thaum lub sij hawm gel electrophoresis los txiav txim ob qhov loj thiab rau kom muaj nuj nqis ntawm PCR cov khoom.
Puas yog DNA tsis zoo lossis zoo?
Cov DNA molecules muaj a tsis zoo them vim yog cov phosphate pawg hauv lawv cov qab zib-phosphate qaum, yog li lawv pib txav los ntawm cov matrix ntawm cov gel mus rau zoo ncej.
Pom zoo:
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