Kev khi isotherm yog dab tsi?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Cov kev khi isotherm (BI) ib kev khi qhov system yog Ameslikas hu ua ib qho nkhaus ntawm cov nyiaj ligands adsorbed raws li kev ua haujlwm ntawm cov concentration lossis ib nrab siab ntawm ligand ntawm qhov kub thiab txias.

Kuj paub, dab tsi yog qhov ruaj khov siab txhais tau tias?

Qhov me me dissociation tas li , qhov ntau tightly khi lub ligand yog, los yog lub siab dua kev sib raug zoo ntawm ligand thiab protein. Piv txwv li, ib tug ligand nrog ib tug nanomolar (nM) dissociation tas li khi nruj dua rau ib qho protein ntau dua li ligand nrog micromolar (ΜM) dissociation tas li.

Tom qab, lo lus nug yog, dab tsi yog qhov kev ua haujlwm toj roob? Cov Hill muaj nuj nqi Cov kev ntsuas muab ib qho kev ntsuas ntawm ligand affinity rau cov receptor (parameter K hauv Equation 2.4), tab sis Hill ua haujlwm feem ntau siv ib yam nkaus li kwv yees tus naj npawb ntawm ligand molecules uas yuav tsum tau khi cov receptor thiaj li tsim tau cov nyhuv ua haujlwm.

Ua li no, kev sib koom tes yog txiav txim siab li cas?

Kev khi tuaj yeem suav hais tias " kev koom tes "yog tias kev khi ntawm thawj molecule ntawm B mus rau A hloov cov kev khi affinity ntawm qhov thib ob B molecule, ua rau nws ntau dua los yog tsawg dua khi . Hauv lwm lo lus, lub kev khi ntawm B molecules mus rau qhov chaw sib txawv ntawm A tsis yog kev sib koom ua ke ywj pheej.

KD yog dab tsi?

Kd yog qhov sib npaug ntawm qhov sib npaug rau qhov sib npaug sib npaug, nws sib npaug rau Kon / Koff, thiab nws units yog M.