Dab tsi yog theem ib txwm thiab rov qab theem chromatography?
Dab tsi yog theem ib txwm thiab rov qab theem chromatography?

Video: Dab tsi yog theem ib txwm thiab rov qab theem chromatography?

Video: Dab tsi yog theem ib txwm thiab rov qab theem chromatography?
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Hauv ib txwm - theem chromatography , qhov chaw nres tsheb theem yog polar thiab mobile theem yog nonpolar. Hauv thim rov qab theem peb muaj qhov opposite; qhov chaw nres tsheb theem yog nonpolar thiab mobile theem yog polar.

Ib yam li ntawd, tib neeg nug, qhov txawv ntawm theem ib txwm thiab rov qab theem chromatography yog dab tsi?

Nyob rau theem ib txwm , polar molecules elute maj mam, thiab tsis-polar (greasy) molecules elute sai. Rov qab theem yog qhov tseem ceeb qhov opposite ntawm ib txwm - theem . Nyob rau theem rov qab , polar molecules elute sai sai, thiab non-polar (greasy) molecules elute maj mam.

Ib yam li ntawd, rov qab theem txhais li cas? Rov qab - theem chromatography yog cov Lub sij hawm muab rau chromatographic tej yam kev mob nyob rau hauv uas ib tug nonpolar nyob ruaj ruaj theem yog siv ua ke nrog ib tug polar mobile theem.

Hauv qhov no, dab tsi yog theem kab kem chromatography?

Ib txwm - theem chromatography (NPC) a chromatographic hom uas siv polar nyob ruaj ruaj theem thiab ib tug nonpolar mobile theem rau kev sib cais ntawm cov polar compounds.

Lub sijhawm rov qab chromatography ua haujlwm li cas?

Rov qab - theem chromatography yog ib qho txheej txheem siv alkyl chains covalently bonded rau qhov chaw nres tsheb theem particles nyob rau hauv thiaj li yuav tsim ib tug hydrophobic nyob ruaj ruaj theem , uas muaj zog affinity rau hydrophobic los yog tsawg polar compounds. Rov qab - theem chromatography ntiav ib tug polar (aqueous) mobile theem.

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