Yam twg polymerase tsis xav tau primer?
Yam twg polymerase tsis xav tau primer?

Video: Yam twg polymerase tsis xav tau primer?

Video: Yam twg polymerase tsis xav tau primer?
Video: Kev hlub tsis ncaj ncees.10/15/2017 2024, Kaum ib hlis
Anonim

RNA polymerase II, lub enzyme uas synthesizes mRNA los ntawm DNA, yeej tsis xav tau ib tug primer.

Raws li txoj cai, vim li cas RNA polymerase tsis xav tau primer?

RNA primers yuav tsum tau pib replication vim DNA polymerase ua tsis tau ua nws ib leeg. DNA kev sau ntawv tsis muaj ib yam teeb meem vim RNA polymerase muaj peev xwm pib RNA synthesis.

Tsis tas li ntawd, qhov twg polymerase tsis xav tau tus qauv? Terminal deoxynucleotidyl transferase (TdT), yog tus qauv-ywj siab DNA polymerase uas catalyzes kev koom ua ke ntawm deoxynucleotides ntawm 3'-hydroxyl terminus ntawm DNA, nrog rau kev tso tawm ntawm inorganic phosphate. TdT tsis xav tau tus qauv thiab yuav tsis luam ib qho.

Hauv qhov no, DNA polymerase 1 puas xav tau primer?

Nyob rau hauv thiaj li yuav DNA polymerization ua qhov chaw, ob qhov yuav tsum tau ua. Ua ntej tshaj plaws, tag nrho DNA polymerases yuav tsum muaj ob lub template strand thiab a primer txoj hlua. Tsis zoo li RNA , DNA polymerases tsis tuaj yeem ua ke DNA los ntawm ib tug template strand.

Dab tsi yog txhais los ntawm primer thiab vim li cas primers tsim nyog rau DNA replication?

tus DNA Ob chav helix yog tuav ua ke los ntawm ob hom kev sib cog lus, covalent thiab hydrogen. Primers yog xav tau vim tus loj DNA polymerase koom nrog DNA replication tsis muaj peev xwm pib DNA synthesis thiab, theej, yuav tsum muaj 3' kawg. (Nws yog pawg 3' -OH uas yog xav tau los tsim cov phosphodiester tom ntej.)

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