Video: Cov ntsev ua li cas rau kev sib xyaw ntawm tes?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Crushing lub kiwi / strawberry txiv hmab txiv ntoo lub cev tawg lub cell phab ntsa. Vim li cas ua peb siv tshuaj zawv plaub hau? Tom qab lub cell phab ntsa tau raug cuam tshuam thaum kho tshuab mashing ntawm cov txiv hmab txiv ntoo, cov tshuaj ntxuav tes hauv cov tshuaj zawv plaub hau cuam tshuam rau cell thiab nuclear membranes ntawm txhua tus cell kom tso DNA.
Ib yam li ntawd, lub hom phiaj ntawm kev ntxiv ntsev hauv DNA extraction yog dab tsi?
Lub luag hauj lwm ntawm cov ntsev yog neutralize tus nqi ntawm cov DNA cov qab zib phosphate qaum. Ethanol muaj qis dielectric tas li tshaj dej yog li nws tau siv los txhawb ionic bonds ntawm Na+ (los ntawm ntsev ) thiab PO3- (los ntawm DNA qaum) ua rau lub DNA los nag.
Vim li cas DNA ua rau hauv ethanol thiab ntsev? DNA yog polar vim nws cov phosphate qaum qaum heev. Yog txaus ethanol ntxiv, cov hluav taws xob attraction ntawm phosphate pawg thiab tej yam zoo ions tam sim no nyob rau hauv kev daws yuav muaj zog txaus los tsim ionic bonds ruaj khov thiab DNA nag lossis daus . Qhov no feem ntau tshwm sim thaum ethanol sau ntau tshaj 64% ntawm cov tshuaj.
Ntawm no, 4 cov kauj ruam yooj yim rau kev rho tawm DNA yog dab tsi?
Cov DNA extraction txheej txheem dawb DNA los ntawm lub cell thiab ces cais nws los ntawm cellular kua thiab proteins ces koj tshuav nrog ntshiab DNA . Peb cov kauj ruam yooj yim ntawm DNA rho tawm yog 1) lysis, 2) nag lossis daus, 3) purification.
Lub hauv paus ntawm DNA Extraction
- Kauj ruam 1: Lysis.
- Kauj Ruam 2: Dej nag.
- Kauj Ruam 3: Purification.
Vim li cas 70 Ethanol siv hauv DNA cais?
DNA yog ntxuav nrog 70 % ethanol tshem tawm ib co (los yog tag nrho cov) ntawm ntsev los ntawm pellet. vim nag hmo hauv 100% ethanol ua kom tshem tawm tag nrho cov dej molecule los ntawm DNA thiab ua kom tiav Lub cev qhuav dej, uas ua rau lawv tsis soluble, Yog li peb muab 70 % ntxuav kom nws khaws qee cov dej molecule thaum ua kom nws soluble.
Pom zoo:
Kev sib xyaw cov khoom xyaw puas yog kev hloov tshuaj?
Cov ntaub ntawv yooj yim ntawm kev sib cais thiab sib xyaw yog suav tias yog kev hloov pauv ntawm lub cev, tab sis kev sib xyaw cov khoom xyaw ntawm lub ncuav mog qab zib tsis yog ib qho kev sib xyaw yooj yim. Kev hloov tshuaj pib tshwm sim thaum cov khoom xyaw sib xyaw, tsim cov tshuaj tshiab
Puas yog txoj cai ntawm kev sib xyaw ua ke siv tau rau cov khoom sib xyaw?
Cov teeb meem tsis tuaj yeem tsim los yog rhuav tshem, txawm hais tias itis ib qho khoom, qhov sib xyaw, lossis sib xyaw. b) Txoj cai lij choj ntawm qhov tseeb muaj pes tsawg leeg tsuas yog siv rau cov khoom sib txuas nkaus xwb, vim hais tias nws hais txog qhov tsis tu ncua, lossis qhov tseeb, muaj pes tsawg leeg ntawm cov ntsiab lus hauv ib qho chaw
Dab tsi yog qhov sib xyaw ntawm cov khoom sib xyaw?
Ib lub tebchaw muaj cov atom ntawm cov khoom sib txawv ntawm cov tshuaj sib xyaw ua ke hauv qhov sib piv ruaj khov. Kev sib tov yog ib qho kev sib xyaw ntawm ob lossis ntau yam tshuaj uas tsis muaj tshuaj lom neeg ua ke lossis cov tshuaj tiv thaiv. Kev sib xyaw. Cov tshuaj muaj cov ntsiab lus sib txawv hauv qhov sib piv ruaj khov uas tau hais tseg los ntawm cov ntawv cog lus tshuaj
Dab tsi cuam tshuam rau kev tshem cov choj ntsev muaj rau kev ua haujlwm ntawm txhua lub xov tooj ntawm tes electrochemical?
Yog tsis muaj ntsev choj, cov tshuaj nyob rau hauv lub anode compartment yuav ua tau zoo them thiab cov tshuaj nyob rau hauv lub cathode compartment yuav ua tsis zoo nqi, vim hais tias ntawm tus nqi imbalance, lub electrode cov tshuaj tiv thaiv yuav sai sai los mus rau ib tug nres, yog li ntawd nws yuav pab kom tswj tau qhov khiav. los ntawm electrons
Dab tsi yog txoj cai ntawm kev sib xyaw rau cov khoom sib xyaw?
Rule of Mixtures yog ib txoj hauv kev rau kev kwv yees kwv yees ntawm cov khoom siv sib xyaw, raws li kev xav tias cov khoom sib xyaw yog qhov ntim hnyav nruab nrab ntawm cov theem (matrix thiab dispersed theem) cov khoom