Video: Dab tsi yog rho dependent termination?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Rho dependent termination yog ib qho ntawm ob hom kev txiav txim nyob rau hauv prokaryotic transcription, lwm yam yog intrinsic (los yog Rho - ywj siab). Tom qab khi rau cov saw tshiab RNA, ρ yam txav mus raws cov molecule hauv 5'-3' kev taw qhia thiab txhawb kev sib cais los ntawm DNA qauv thiab RNA polymerase.
Yog li, qhov txawv ntawm Rho nyob thiab kev txiav tawm ywj siab yog dab tsi?
Intrinsic (los yog rho - ywj siab ) kev txiav txim yog thaum RNA tsim cov qauv plaub hau uas hloov RNA Polymerase thiab nres cov ntawv sau. Rho - nyob ntawm kev txiav txim tshwm sim thaum lub rho protein disassociates RNA Polymerase thiab txav nws tawm ntawm lub template.
Tsis tas li ntawd, eukaryotes puas muaj rho tawm qhov kev txiav tawm? Eukaryotes daim ntawv thiab pib complex nrog ntau yam kev hloov pauv uas cuam tshuam tom qab pib ua tiav. Eukaryotes muaj mRNAs uas yog monocystronic. Kev txiav txim hauv prokaryotes yog ua los ntawm ob qho tib si rho - nyob los yog rho - ywj siab mechanisms.
Tsuas yog li ntawd, rho tawm qhov kev txiav txim siab rho tawm dab tsi yuav tsum tau ua?
Escherichia coli protein Rho yog xav tau rau Factor- dependent transcription termination los ntawm RNA polymerase thiab yog qhov tseem ceeb rau kev ua haujlwm ntawm lub cell. Nws yog homohexameric protein uas paub txog thiab khi zoo dua rau C-nplua nuj qhov chaw nyob hauv luam tawm RNA.
Dab tsi muab lub zog rau Rho nyob ntawm cov saw txiav?
Rho - nyob ntawm kev txiav txim tshwm sim los ntawm kev khi ntawm Rho rau ribosome-free mRNA, C-nplua nuj qhov chaw yog cov neeg sib tw zoo rau kev khi. Rho's ATPase yog qhib los ntawm Rho -mRNA khi, thiab muab lub zog rau Rho translocation raws mRNA; translocation yuav tsum zawv zawg ntawm cov lus mus rau hauv lub hauv paus qhov ntawm lub hexamer.
Pom zoo:
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Dab tsi yog qhov txawv ntawm Rho dependent thiab kev txiav tawm ywj siab?
Intrinsic (los yog rho-independent) kev txiav tawm yog thaum RNA tsim cov qauv plaub hau uas hloov RNA Polymerase thiab nres cov ntawv sau. Rho-dependent termination tshwm sim thaum rho protein disassociates RNA Polymerase thiab txav nws tawm ntawm lub template
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