Dab tsi yog qhov zoo ntawm agarose tshaj polyacrylamide gels?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Cov gels tuaj yeem siv los daws qhov tawg tawg loj ntawm DNA. Polyacrylamide gels yog siv los cais luv nucleic acids, feem ntau nyob rau hauv thaj tsam ntawm 1-1000 puag khub, raws li ntawm cov concentration siv (Daim duab 1). Cov no cov gels tuaj yeem khiav nrog lossis tsis muaj denaturant.

Tsis tas li ntawd, qhov txawv ntawm agarose thiab polyacrylamide gels yog dab tsi?

Agarose muaj ntau molecules, thaum polyacrylamide feem ntau muaj tsuas yog ib qho loj molecule. Lub molecule ntawm polyacrylamide yog tsim los ntawm DNA lossis protein. Qhov khoob nruab nrab tus cov gels ntawm polyacrylamide me dua cov nruab nrab tus cov gels ntawm agarrose , uas yog lwm tus qhov sib txawv ntawm ob yam no.

Vim li cas peb thiaj siv polyacrylamide gel? Polyacrylamide gel electrophoresis yog ib lub cuab yeej muaj zog siv los soj ntsuam RNA cov qauv. Thaum twg polyacrylamide gel yog denatured tom qab electrophoresis, nws muab cov ntaub ntawv ntawm tus qauv muaj pes tsawg leeg ntawm RNA hom.

Hais txog qhov no, qhov zoo ntawm gel electrophoresis yog dab tsi?

Cov qhov zoo yog cov gel yog yooj yim poured, tsis denature cov qauv. Cov qauv kuj tuaj yeem rov qab tau. Qhov tsis zoo yog qhov ntawd cov gels tuaj yeem yaj thaum lub sijhawm electrophoresis , qhov tsis tuaj yeem ua rau qaug zog, thiab cov ntaub ntawv sib txawv ntawm cov khoom siv caj ces yuav khiav hauv cov ntaub ntawv tsis paub tseeb.

Polyacrylamide gel electrophoresis txawv ntawm agarose gel electrophoresis li cas?

Agarose yog complex thiab muaj qhov sib txawv ntawm ntau qhov sib txawv me me molecules uas ua rau gel matrix. Polyacrylamide yog ua los ntawm tsuas yog ib hom loj molecular, uas muaj qhov sib txawv me me, txawm tias qhov loj me tuaj yeem sib txawv. Agarose yog nchuav horizontally, thiab polyacrylamide yog nchuav vertically.