Video: TFA yog dab tsi hauv chemistry?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Trifluoroacetic acid ( TFA ) yog ib qho organofluorine compound nrog rau tshuaj Cov mis mos CF3CO2H. Nws yog cov qauv sib piv ntawm acetic acid nrog tag nrho peb ntawm acetyl pawg hydrogen atoms hloov los ntawm fluorine atoms thiab yog cov kua tsis muaj xim nrog cov kua qaub xws li tsw.
Hauv qhov no, vim li cas TFA siv hauv HPLC?
TFA ( trifluoroacetic acid ) yog feem ntau siv mobile theem additive rau reversed-theem HPLC (RP- HPLC ) kev sib cais ntawm cov protein thiab peptides. Txawm li cas los xij, TFA cuam tshuam nrog thiab txo qis LC / MS teeb liab, txo qhov rhiab heev.
Tom qab, lo lus nug yog, yog TFA soluble hauv dej? TFA yog yuav luag 100,000-fold ntau acidic dua acetic acid . TFA yog dav siv nyob rau hauv cov organic chemistry. Trifluoroacetic acid zoo nkaus li cov xim tsis muaj xim fuming nrog cov ntxhiab tsw. Soluble nyob rau hauv dej thiab denser tshaj dej.
Kuj paub, yog TFA lom?
Kev nyab xeeb. Trifluoroacetic acid yog ib qho corrosive acid tab sis nws tsis ua rau muaj kev phom sij cuam tshuam nrog hydrofluoric acid vim tias carbon-fluorine daim ntawv cog lus tsis yog labile. TFA yog teeb meem thaum nqus tau, ua rau tawv nqaij kub hnyiab thiab yog tshuaj lom rau cov kab mob hauv dej txawm tias tsis tshua muaj siab.
Kuv yuav ua li cas tshem TFA los ntawm cov tshuaj tiv thaiv?
Nrov teb (1) Rau tshem tawm kab ntawm TFA koj tuaj yeem siv exsiccator nrog KOH thiab - xaiv tau - qee qhov cua sov. Yog tias koj muaj ntsev nrog TFA Koj tuaj yeem yaj koj cov khoom hauv dej ntxiv qee qhov NH3 - kom txog thaum koj muaj cov kab mob alkalline me ntsis - thiab rho tawm koj cov khoom nrog CHCl3 lossis DCM, evaporate thiab qhuav dhau KOH.
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