Video: Vim li cas nitrocellulose membrane siv nyob rau yav qab teb blotting?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nyob rau hauv thawj txoj cai nitrocellulose daim nyias nyias ua tau siv rau cov blotting li cas Southern blot tab sis nyob rau lub sijhawm tsis ntev los no nylon daim nyias nyias tau muab coj los siv rau blotting txheej txheem vim lawv muaj peev xwm los khi ntau npaum li cas ntawm DNA zoo uas tso cai rau cov Southern blot yuav tsum tau nqa nrog tsawg npaum li cas ntawm
Yog li, vim li cas nitrocellulose membrane siv rau blotting?
Nitrocellulose daim nyias nyias yog matrix nrov siv hauv protein blotting vim lawv cov protein ntau-binding affinity, compatibility nrog ntau yam kev tshawb nrhiav (chemiluminescence, chromogenic, thiab fluorescence), thiab muaj peev xwm immobilize proteins, glycoproteins, los yog nucleic acids.
Tsis tas li ntawd, vim li cas DNA hloov mus rau ib lub nylon membrane? Los ntawm kev siv 0.4 M NaOH ua tus hloov hnyav tom qab kev kho mob luv luv ntawm cov gel hauv cov kua qaub, DNA yog depurinated thaum lub sij hawm hloov . Kev nphav raug ntawm DNA mus rau ultraviolet lub teeb, nyob rau hauv lub gel los yog raws li nram no hloov rau nylon membrane , txo nws lub peev xwm hybridize.
Tsis tas li ntawd, lub hom phiaj ntawm Southern blotting yog dab tsi?
A Southern blot yog ib txoj hauv kev siv hauv molecular biology txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau kuaj DNA. Southern blotting ua ke hloov pauv ntawm electrophoresis-sib cais DNA tawg mus rau lub lim membrane thiab tom qab nrhiav kom pom cov tawg los ntawm kev sojntsuam hybridization.
Southern blotting pab hauv kev tshawb fawb forensic li cas?
A Southern blot , npe tom qab nws inventor Edwin Qab teb , yog ib txoj hauv kev siv los kuaj xyuas qhov muaj cov DNA ib ntus hauv cov qauv DNA. Thaum ib tus neeg DNA tau zom nrog ib qho kev txwv tshwj xeeb, cov khoom tawg tau raug tshuaj xyuas los ntawm Southern blot tsom xam.
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