Video: Coomassie xiav siv rau hauv SDS PAGE yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Qhov no puas pab tau?
Yog · Tsis yog
Ntxiv mus, vim li cas Coomassie xiav siv hauv SDS PAGE?
Coomassie Blue stain yog siv rau stain cov protein bands hauv polyacrylamide gels. Cov dyes khi nruj dua rau cov protein ntau dua li cov gel matrix, txawm li cas los xij, yog li cov xim tom qab tuaj yeem raug tshem tawm ntawm tsuas yog cov khoom tsis muaj protein ntau ntawm cov gel siv cov kuab tshuaj zoo sib xws uas cov dye raug tshem tawm. Qhov no yog lub destain.
Ib yam li ntawd, cov amino acids dab tsi Coomassie xiav khi rau? Nyob rau hauv acidic tej yam kev mob, cov dyes khi rau cov proteins feem ntau los ntawm cov amino acids yooj yim (feem ntau. arginine , lysine thiab histidine ), thiab tus naj npawb ntawm coomassie dye ligands khi rau txhua tus protein molecule yog kwv yees li proportional rau tus naj npawb ntawm cov nqi zoo pom ntawm cov protein.
Ib sab saum toj no, lub luag haujlwm ntawm bromophenol xiav hauv SDS PAGE yog dab tsi?
Nws yog feem ntau siv raws li kev taug qab zas xim thaum lub sij hawm agarose los yog polyacrylamide gel electrophoresis. Bromophenol xiav muaj qhov tsis zoo me ntsis thiab yuav txav mus rau tib qho kev taw qhia zoo li DNA, tso cai rau tus neeg siv los saib xyuas qhov kev nce qib ntawm cov molecules txav mus los gel . Tus nqi ntawm kev tsiv teb tsaws txawv nrog gel muaj pes tsawg leeg.
Lub luag haujlwm ntawm SDS hauv SDS PAGE yog dab tsi?
Nws siv sodium dodecyl sulfate (SDS) molecules los pab txheeb xyuas thiab cais tawm protein ntau molecules. SDS-PAGE yog ib lub tshuab electrophoretic tsis tu ncua tsim los ntawm Ulrich K. Laemmli uas feem ntau siv los ua ib txoj hauv kev los cais cov proteins nrog molecular masses ntawm 5 thiab 250 KDa.
Pom zoo:
Cov xim dab tsi Bromothymol xiav tig rau hauv cov tshuaj nruab nrab?
Cov kev siv tseem ceeb ntawm bromothymol xiav yog rau kev kuaj pH thiab kuaj cov photosynthesis thiab ua pa. Bromothymol xiav muaj xim xiav thaum nyob rau hauv tej yam kev mob (pH tshaj 7), xim ntsuab nyob rau hauv nruab nrab tej yam kev mob (pH ntawm 7), thiab daj xim nyob rau hauv acidic tej yam kev mob (pH nyob rau hauv 7)
Dab tsi yog qhov sib npaug siv los xam tag nrho cov khoom siv hluav taws xob siv?
Cov mis uas txuas lub zog thiab lub zog yog: Zog = Lub Zog x Sijhawm. Chav tsev ntawm lub zog yog joule, lub zog ntawm lub zog yog watt, thiab chav tsev ntawm lub sijhawm yog qhov thib ob
Lub neej ntawm lub hnub qub xiav xiav yog dab tsi?
Lub hnub qub nruab nrab zoo li peb lub hnub yuav kav ntev txog 12 billion xyoo, thaum lub ntuj xiav supergiant yuav tawg nrog ob peb puas lab xyoo
Lub hom phiaj ntawm kev siv Bromothymol xiav hauv O F test yog dab tsi?
Cov kev siv tseem ceeb ntawm bromothymol xiav yog rau kev kuaj pH thiab kuaj cov photosynthesis thiab ua pa. Bromothymol xiav muaj xim xiav thaum nyob rau hauv tej yam kev mob (pH tshaj 7), xim ntsuab nyob rau hauv nruab nrab tej yam kev mob (pH ntawm 7), thiab daj xim nyob rau hauv acidic tej yam kev mob (pH nyob rau hauv 7)
Lub ntuj puas xiav vim dej hiav txwv los yog dej hiav txwv xiav vim lub ntuj?
'Lub hiav txwv zoo li xiav vim liab, txiv kab ntxwv thiab daj (ntev wavelength teeb) yog absorbed ntau zog los ntawm dej tshaj yog xiav (luv wavelength light). Yog li thaum lub teeb dawb ntawm lub hnub nkag mus rau hauv dej hiav txwv, nws feem ntau yog xiav uas tau rov qab los. Ib yam li ntawd, lub ntuj xiav.'