Internal positive control txhais li cas?
Internal positive control txhais li cas?

Video: Internal positive control txhais li cas?

Video: Internal positive control txhais li cas?
Video: How to motivate yourself to change your behavior | Tali Sharot | TEDxCambridge 2024, Kaum ib hlis
Anonim

Internal Positive Controls yog muab rho tawm ib txhij thiab/los yog amplified nyob rau hauv tib lub raj nrog lub hom phiaj pathogen thiab, ua ke nrog ib tug kev tswj zoo , ua pov thawj kev ua haujlwm ntawm cov tshuaj tiv thaiv sib xyaw kom raug amplification ntawm lub hom phiaj pathogen.

Hais txog qhov no, dab tsi yog kev tswj zoo hauv PCR?

Ob leeg zoo thiab kev tswj tsis zoo yog siv rau hauv PCR kev sim. Cov kev tswj zoo , ib qho piv txwv paub txog cov kab mob DNA, qhia tau hais tias cov primers tau txuas rau DNA strand. Cov kev tswj tsis zoo , ib qho qauv uas tsis muaj DNA, qhia tau hais tias muaj kab mob ntawm cov kab mob PCR sim nrog txawv teb chaws DNA tau tshwm sim.

Vim li cas koj thiaj xav tau kev tswj xyuas qhov zoo thiab tsis zoo thaum khiav lub gel? A kev tswj zoo tau txais kev kho mob nrog cov lus teb paub, kom qhov no zoo cov lus teb tuaj yeem muab piv rau cov lus teb tsis paub txog kev kho mob. Qhov no yog siv hauv electrophoresis los sib piv cov DNA strands rau DNA Standard. Cov kev tswj tsis zoo yog siv thaum tsis xav tau teb.

Ib yam li ntawd, dab tsi yog kev tswj hwm sab hauv hauv PCR?

Internal tswj yog siv raws li qhov taw qhia ntawm zoo meej nucleic acid extraction, zoo ntawm cov qauv, zoo ntawm PCR . Piv txwv li, nyob rau hauv cov ntaub ntawv ntawm kev soj ntsuam kuaj los ntawm tib neeg, nrhiav tau ntawm ib co noob nrog Ct qhov tseem ceeb nyob rau hauv ib tug ntau yam yuav qhia tau hais tias cov qauv tau sau / thauj / khaws cia zoo.

Dab tsi yog lub hom phiaj ntawm kev tswj kom muaj zog hauv PCR?

Ib sab hauv amplification tswj (IAC) yog ib hom DNA uas tsis muaj hom phiaj tam sim no nyob rau hauv tib lub raj raws li cov qauv, uas yog sib xyaw ua ke nrog cov phiaj xwm sib lawv liag. Ib qho IAC yuav tsum tau tiv thaiv cov txiaj ntsig tsis zoo uas yuav tshwm sim los ntawm PCR inhibitors (Radstrom et al. 2003).

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