Video: Yuav ua li cas txawv teb chaws DNA muab tso rau hauv hlwb?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Transduction yog kev ntxig ntawm txawv teb chaws DNA rau hauv a cell ntawm tus kab mob (Saib Daim Ntawv Qhia 1 thiab 2). Cov kab mob no yog ua los ntawm lub tsho tiv thaiv protein uas nyob hauv tsev DNA hauv. Kab mob ua tau khi rau nyob hlwb thiab txhaj lawv DNA . Los yog, kab mob ua tau thawb rau hauv tus tswv tsev raws li ib tug membrane-bound vesicle, ua ntej tso lawv DNA hauv tus tswv tsev.
Tsuas yog li ntawd, puas yog txhua txoj hauv kev los ntxig cov DNA txawv teb chaws rau hauv cov hlwb?
Muaj ntau txoj hauv kev DNA txawv teb chaws tuaj yeem qhia tau mus rau hauv cov hlwb suav nrog kev hloov pauv, hloov pauv, sib txuas, thiab hloov pauv. Transformation, transduction, thiab conjugation tshwm sim nyob rau hauv cov ntaub ntawv ntawm HGT, tab sis transfection yog tshwj xeeb rau lub lab. Cia wb mus saib qhov txawv no txoj kev ntawm DNA kev ntxig.
Tsis tas li ntawd, dab tsi yog plasmid nrog txawv teb chaws DNA tso? Plasmids zoo ib yam li cov kab mob, tab sis tsis muaj lub tsho tiv thaiv protein thiab tsis tuaj yeem txav los ntawm lub xov tooj mus rau hauv lub xov tooj ntawm tes tib yam li tus kab mob. Plasmid vectors yog me me ncig molecules ntawm ob stranded DNA muab tau los ntawm ntuj plasmids uas tshwm sim hauv cov kab mob hlwb. Ib tug tshiab plasmid muaj cov DNA txawv teb chaws as ib ntxig yog tau.
Hais txog qhov no, DNA yuav muab tso rau hauv cov kab mob li cas?
Ib zaug ib vector uas muaj txawv teb chaws DNA tau tsim nyob rau hauv lub lab, nws yog qhia ua kab mob hlwb. Cov kws tshawb fawb ua Qhov no los ntawm kev tsim cov qhov me me (pores) hauv lub kab mob cell membrane. Ib zaug kab mob tau rov qab los ntawm cov txheej txheem ntawm kev qhia DNA (hu ua transformation), lawv ua tau yuav tau kab lis kev cai nyob rau hauv lub lab.
Dab tsi yog txawv teb chaws DNA?
txawv teb chaws / neeg nrog caij DNA yog ib feem ntawm DNA molecule uas yog enzymatically cais thiab cloned. Cov noob raug txheeb xyuas ntawm genome thiab rub tawm ntawm nws ua ntej lossis tom qab cloning. Kev txheeb xyuas thiab tus cwj pwm ntawm DNA Cov kab ke yog qhov nyuaj dua ntawm nws cov genome dua li siv mRNA, yog tias nws nyob hauv daim ntawv ntshiab.
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