Qhov nruab nrab liab ua haujlwm li cas?
Qhov nruab nrab liab ua haujlwm li cas?

Video: Qhov nruab nrab liab ua haujlwm li cas?

Video: Qhov nruab nrab liab ua haujlwm li cas?
Video: Mob nrob qaum/ Mob duav yog vim li cas? 2024, Kaum ib hlis
Anonim

Neutral liab yog ib qho eurhodin dye uas stains lysosomes hauv cov hlwb siv tau. Cov hlwb uas muaj peev xwm tuaj yeem nqa tau nruab nrab liab Los ntawm kev thauj mus los thiab koom nrog cov xim rau hauv lawv cov lysosomes tab sis cov hlwb tsis tuaj yeem tsis tuaj yeem nqa cov chromophore no.

Yog li ntawd, qhov nruab nrab liab stain yog dab tsi?

Neutral liab (toluylene liab , Basic Liab 5, sau C. I. 50040) yog eurhodin dye siv rau staining hauv histology. Nws stains cov lysosomes liab . Nws yog siv raws li ib tug general stain nyob rau hauv histology, raws li ib tug counterstain nyob rau hauv ua ke nrog rau lwm yam dyes, thiab rau ntau staining txoj kev.

thaum nruab nrab liab yog muab tso rau hauv ib tug acidic tov nws ua? Nqe lus piav qhia: Neutral liab yog ib qho hydrochloride tau los ntawm kev sib txuas cov dawb puag ntawm nruab nrab liab nrog ib qho sib npaug ntawm hydrochloric acid. Neutral liab ua raws li qhov ntsuas pH, hloov ntawm liab rau daj ntawm pH 6.8 thiab 8.0.

Tsis tas li ntawd, koj yuav ua li cas thiaj ua tau ib qho liab nruab nrab?

  1. Kev npaj ua piv txwv: a. Rau cov hlwb hauv kab lis kev cai: Kho cov hlwb nrog 4% paraformaldehyde rau 10 ~ 20 feeb, thiab yaug ob zaug nrog dej distilled rau 2 ~ 5 feeb. b.
  2. Neutral Red staining: Ntxiv Cov Tshuaj Liab Tsis Txaus Siab (Cov Khoom A) thiab ua rau 2 ~ 10 feeb ntawm chav tsev kub.

Vim li cas liab nruab nrab ntxiv rau cov qauv poov xab?

Xim Hloov Thauj ntawm NH3 rau hauv poov xab cell ua rau nruab nrab liab stain tig daj nyob rau hauv lub xub ntiag ntawm lub hauv paus.

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