Qhov loj spectrometry qhia tau hais tias muaj nyob ntawm isotopes li cas?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Isotopes muaj qhov sib txawv atomic masses. Tus txheeb ze abundance ntawm txhua isotope tau yuav txiav txim siv pawg spectrometry . A loj spectrometer ionizes atoms thiab molecules nrog high-zog electron beam thiab ces deflects cov ions los ntawm ib tug magnetic teb raws li lawv. pawg -rau-them tus nqi (m / z m / z m / z).

Ntawm no, cov ntaub ntawv los ntawm huab hwm coj spectrometry qhia tau hais tias muaj cov isotopes li cas?

Tsis yog, feem ntau cov ntsiab lus muaj nyob rau hauv qhov sib txawv isotopes ntawm tib lub ntsiab. Thaum ib qho piv txwv ntawm cov khoom ntshiab yog soj ntsuam nrog a loj spectrometer , cwb isotope yuav yuav ionized thiab kuaj. Cov pawg spectrum sawv cev rau txhua tus isotope raws li lub ncov, plotting nws pawg kom them (m / z) piv thiab nws cov txheeb ze siv.

Tsis tas li ntawd, koj ua li cas xam qhov nruab nrab loj ntawm ib isotope? Rau xam tus nruab nrab loj , ua ntej hloov cov feem pua rau hauv feem (sib faib lawv los ntawm 100). Ces, xam tus pawg tus lej. Cov tshuaj chlorine isotope nrog 18 neutrons muaj ntau ntawm 0.7577 thiab a pawg npe 35am.

Yog li ntawd, huab hwm coj spectrometry qhia cov tebchaw li cas?

Qhov siab tshaj- pawg ib ion a spectrum yog feem ntau suav hais tias yog cov molecular ion, thiab qis dua- pawg ions yog fragments los ntawm molecular ion, piv txwv li cov qauv yog ib tug ntshiab compound . Txawm tias cov no compounds yog zoo sib xws hauv loj, nws yog ib qho yooj yim teeb meem rau txheeb xyuas lawv los ntawm lawv tus kheej pawg spectrum.

Dab tsi muaj qhov loj ntawm 1 amu?

Lub atomic mass unit (symbolized AMU lossis amu) yog txhais raws li qhov tseeb 1/12 qhov loj ntawm ib qho atom ntawm carbon-12. Cov pa roj carbon-12 (C-12) atom muaj rau protons thiab rau neutrons hauv nws cov nucleus. Hauv cov ntsiab lus tsis meej, ib qho AMU yog qhov nruab nrab ntawm qhov proton so mass thiab neutron so mass.